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首页> 外文期刊>Cell biology international. >Microautophagy involves programmed cell semi-death of sieve elements in developing caryopsis of Triticum aestivum L.
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Microautophagy involves programmed cell semi-death of sieve elements in developing caryopsis of Triticum aestivum L.

机译：微自噬涉及普通小麦发育中的颖果中筛分元件的程序性细胞半死亡。

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摘要

Differentiation of sieve elements (SEs) involves programmed cell semi-death, in which a small amount of organelles is retained. However, the mechanisms by which a large amount of SE cytoplasm is degraded and the specific proteases involved are not clear. In this study, we confirmed that the degradation of cytoplasm outside of the vacuole was mediated by microautophagy of the vacuole, and that the tonoplast selectively fused with the plasma membrane after most of the cytoplasm in the vacuoles was degraded. The integration of space enclosed a small amount of cytoplasm. Therefore, that fraction of the cytoplasm was preserved. At the same time, the cytosol was weakly acidic during membrane fusion because part of the tonoplast was ruptured. We also demonstrated that wheat aspartic protease (WAP1) and proteases including cathepsin B activity (PICA) were involved in programmed cell semi-death of SEs. PICA showed strongest activity before mass of the cytoplasm was degraded, which might contribute toward SE stability. We found that WAP1 mainly degraded the cytoplasm. Therefore, programmed cell semi-death of SEs might result from the joint action of vacuoles and multiple proteases.
机译：筛元素（SEs）的分化涉及程序性细胞半死亡，其中保留了少量的细胞器。但是，大量SE细胞质降解的机制以及所涉及的特定蛋白酶尚不清楚。在这项研究中，我们证实液泡外的细胞质降解是由液泡的微自噬介导的，并且液泡中的大部分细胞质被降解后，液泡膜与质膜选择性融合。空间的整合封闭了少量的细胞质。因此，保留了那部分细胞质。同时，由于部分液泡膜破裂，细胞质在膜融合过程中呈弱酸性。我们还证明，小麦天冬氨酸蛋白酶（WAP1）和蛋白酶包括组织蛋白酶B活性（PICA）参与了SEs的程序性细胞半死亡。 PICA在细胞质降解之前显示出最强的活性，这可能有助于SE的稳定性。我们发现WAP1主要降解细胞质。因此，SE的程序性细胞半死亡可能是由液泡和多种蛋白酶的联合作用引起的。

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