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首页> 外文期刊>Life sciences >Human peritoneal monocytic cells: lipoprotein uptake and foam cell formation.
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Human peritoneal monocytic cells: lipoprotein uptake and foam cell formation.

机译：人腹膜单核细胞：脂蛋白摄取和泡沫细胞形成。

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摘要

Human peritoneal cells isolated from dialysis effluent have in vivo maturated human macrophages that could serve as a model for studying lipoprotein metabolism and foam cell formation. We previously characterized the low density lipoprotein (LDL) and acetylated LDL (acetyl-LDL) receptor activities of human total peritoneal cells. Now, we provide evidence that both LDL and acetyl-LDL stimulate acylCoA cholesterol:acyl transferase (ACAT) activity of peritoneal cells. Prolonged incubation of cells with LDL results in suppression of ACAT activity, while incubation with acetyl-LDL results in elevated and sustained enzyme activity. When human peritoneal cells were analyzed using flow cytometry, the cell population showed reactivity for CD2, CD4, CD8, CD20, CD14 and HLA-DR antigens. Purified human peritoneal mononuclear cells degraded LDL. Human peritoneal macrophages formed foam cells when exposed to LDL or acetyl-LDL in culture, and lipid deposition increased with incubation time. Macrophages incubated in the presence of butylated hydroxy toluene and LDL did not form foam cells.
机译：从透析流出物中分离出的人腹膜细胞具有体内成熟的人巨噬细胞，可作为研究脂蛋白代谢和泡沫细胞形成的模型。我们以前表征了人类总腹膜细胞的低密度脂蛋白（LDL）和乙酰化LDL（乙酰基LDL）受体活性。现在，我们提供证据表明LDL和乙酰基LDL均可刺激腹膜细胞的酰基CoA胆固醇：酰基转移酶（ACAT）活性。用LDL长时间孵育细胞会导致ACAT活性受到抑制，而使用乙酰基LDL孵育会导致酶的活性持续升高。当使用流式细胞仪分析人腹膜细胞时，细胞群显示出对CD2，CD4，CD8，CD20，CD14和HLA-DR抗原的反应性。纯化的人腹膜单核细胞降解了LDL。当暴露于培养物中的LDL或乙酰基LDL时，人类腹膜巨噬细胞会形成泡沫细胞，并且脂质沉积会随着孵育时间的增加而增加。在丁基化羟基甲苯和LDL存在下孵育的巨噬细胞未形成泡沫细胞。

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