• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Glutamine: fructose-6-phosphate amidotransferase activity and gene expression are regulated in a tissue-specific fashion in pregnant rats.
【24h】

Glutamine: fructose-6-phosphate amidotransferase activity and gene expression are regulated in a tissue-specific fashion in pregnant rats.

机译：谷氨酰胺：果糖-6-磷酸酰胺基转移酶的活性和基因表达以组织特异性方式在妊娠大鼠中受到调节。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We examined whether regulation of glutamine: fructose-6-phosphate amidotransferase (GFA), the rate-limiting enzyme of the hexosamine pathway, is tissue specific and if so whether such regulation occurs at the level of gene expression. We compared GFA activity and expression and levels of UDP-hexosamines and UDP-hexoses between insulin-sensitive (liver and muscle) tissues and a glucose-sensitive (placenta) tissue from 19 day pregnant streptozotocin diabetic and non-diabetic rats. In pregnant non-diabetic rats GFA activities averaged (1521+/-75 pmol/mg protein x min) in the placenta, 895+/-74 in the liver and 81+/-11 in muscle (p<0.001 between each tissue). In the diabetic rats, GFA activities were approximately 50% decreased both in the liver (340+/-42 pmol/mg protein x min, p<0.05 vs control rats) and in skeletal muscle (46+/-3, p<0.05) compared to control rats. In the placenta, GFA activities were identical between diabetic (1519+/-112 pmol/mg protein x min) and non-diabetic (1521+/-75) animals. In the liver, the reduction in GFA activity could be attributed to a significant decrease in GFA mRNA concentrations, while GFA mRNA concentrations were similar in the placenta between diabetic and non-diabetic animals. UDP-N-acetylglucosamine (UDP-GlcNAc), the end product of the hexosamine pathway, was significantly reduced in the liver and in skeletal muscle but similar in the placenta between diabetic and non-diabetic rats. In summary, GFA activity and expression and the concentration of UDP-GlcNAc are decreased in the liver but unaltered in the placenta, although GFA activity is almost 2-fold higher in this tissue than in the liver. These data provide the first evidence for tissue specific regulation of GFA and for its regulation at the level of gene expression.
机译：我们检查了谷氨酰胺：6-磷酸果糖酰胺基转移酶（GFA）（己糖胺途径的限速酶）的调节是否是组织特异性的，如果是，则这种调节是否发生在基因表达水平上。我们比较了来自19天怀孕的链脲佐菌素糖尿病和非糖尿病大鼠的胰岛素敏感组织（肝脏和肌肉）和葡萄糖敏感组织（胎盘组织）的GFA活性以及UDP-己糖胺和UDP-己糖的表达及水平。在怀孕的非糖尿病大鼠中，胎盘中的GFA活性平均（1521 +/- 75 pmol / mg蛋白x分钟），肝脏中的GFA活性平均（895 +/- 74），肌肉中的GFA活性平均（每个组织之间p <0.001） 。在糖尿病大鼠中，肝脏（340 +/- 42 pmol / mg蛋白x分钟，与对照组大鼠相比，p <0.05）和骨骼肌（46 +/- 3，p <0.05）中的GFA活性均降低了约50％。 ）与对照组相比。在胎盘中，糖尿病动物（1519 +/- 112 pmol / mg蛋白x分钟）和非糖尿病动物（1521 +/- 75）的GFA活性相同。在肝脏中，GFA活性的降低可能归因于GFA mRNA浓度的显着降低，而糖尿病动物和非糖尿病动物的胎盘中GFA mRNA的浓度相似。己糖胺途径的最终产物UDP-N-乙酰氨基葡萄糖（UDP-GlcNAc）在肝脏和骨骼肌中明显减少，但在糖尿病和非糖尿病大鼠的胎盘中相似。总之，肝脏中的GFA活性和表达以及UDP-GlcNAc的浓度均降低，但胎盘中未改变，尽管该组织中的GFA活性几乎比肝脏高2倍。这些数据为GFA的组织特异性调控及其在基因表达水平的调控提供了第一个证据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号