...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Changes in distribution of basic nuclear proteins and chromatin organization during spermiogenesis in the greater bandicoot rat, Bandicota indica.
【24h】

Changes in distribution of basic nuclear proteins and chromatin organization during spermiogenesis in the greater bandicoot rat, Bandicota indica.

机译：更大的班迪科大rat，Bandicota indica，在生精过程中基本核蛋白分布和染色质组织的变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Male germ cells of the greater bandicoot rat, Bandicota indica, have recently been categorized into 12 spermiogenic steps based upon the morphological appearance of the acrosome and nucleus and the cell shape. In the present study, we have found that, in the Golgi and cap phases, round spermatid nuclei contain 10-nm to 30-nm chromatin fibers, and that the acrosomal granule forms a huge cap over the anterior pole of nucleus. In the acrosomal phase, many chromatin fibers are approximately 50 nm thick; these then thickened to 70-nm fibers and eventually became 90-nm chromatin cords that are tightly packed together into highly condensed chromatin, except where nuclear vacuoles occur. Immunocytochemistry and immunogold localization with anti-histones, anti-transition protein2, and anti-protamine antibodies suggest that histones remain throughout spermiogenesis, that transition proteins are present from step 7 spermatids and remain until the end of spermiogenesis, and that protamines appear at step 8. Spermatozoa from the cauda epididymidis have been analyzed by acid urea Triton X-100 polyacrylamide gel electrophoresis for basic nuclear proteins. The histones, H2A, H3, H2B, and H4, transitional protein2, and protamine are all present in sperm extracts. These findings suggest that, in these sperm of unusual morphology, both transition proteins and some histones are retained, a finding possibly related to the unusual nuclear form of sperm in this species.
机译：最近，根据顶体和核的形态以及细胞形状，大手足ico大鼠（Bandicota indica）的雄性生殖细胞被分为12个生精步骤。在本研究中，我们发现，在高尔基体和帽状相中，圆形精子核包含10nm至30nm的染色质纤维，顶体颗粒在核前极上方形成一个巨大的帽状。在顶体相中，许多染色质纤维约为50 nm厚;然后将它们增粗到70 nm的光纤，最后变成90 nm的染色质线，紧紧地包装在一起成为高度浓缩的染色质，除非出现核液泡。免疫细胞化学和具有抗组蛋白，抗过渡蛋白2和抗鱼精蛋白抗体的免疫金定位表明，组蛋白在整个精子发生过程中都保留着，从步骤7的精子细胞中存在过渡蛋白，一直保留到精子发生结束，并且在步骤8出现鱼精蛋白。用酸性尿素Triton X-100聚丙烯酰胺凝胶电泳分析了附睾马尾精子中的碱性核蛋白。组蛋白H2A，H3，H2B和H4，过渡蛋白2和鱼精蛋白都存在于精子提取物中。这些发现表明，在这些形态异常的精子中，过渡蛋白和一些组蛋白都被保留了，这一发现可能与该物种精子的异常核形式有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号