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首页> 外文期刊>Life sciences >Differential regulation of intracellular Ca~(2+) signalling induced by high K~+ and endothelin-1 in single smooth muscle cells of intact canine basilar artery: detection by means of confocal laser microscopy
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Differential regulation of intracellular Ca~(2+) signalling induced by high K~+ and endothelin-1 in single smooth muscle cells of intact canine basilar artery: detection by means of confocal laser microscopy

机译：高K〜+和内皮素-1在完整犬基底动脉单个平滑肌细胞中诱导的细胞内Ca〜（2+）信号转导的差异调节

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Changes in intracellular calcium concentration ([Ca~(2+)]i) in smooth muscle cells play the key role in regulation of vascular smooth muscle tone and pathogenesis of cerebral vasospasm. In this study, we adopted the confocal laser microscopy to detect the fluorescence signals arising from the individual smooth muscle cells of canine basilar artery. Ring preparations were made, loaded with fluo-3 and changes in fluorescence induced by high K~+ and endothelin-1 (ET-1) were measured by confocal laser microscopy. In some unstimulated smooth muscle cells Ca~(2+) waves arising from discrete region of the cell propagated to the whole cell with a velocity of approx 10 #mu#m/s. High K~+ (80 mmol/L) induced a rapid rise in [Ca~(2+)]i, the peak level being consistently reached approximately 10 s after stimulation. In contrast, the time to peak level of [Ca~(2+)]i induced by ET-1 (0.3 #mu#mol/L) varied widely between 13 and 26 s among individual cells, an indication that the extent of nonuniform coordination of increases in [Ca~(2+)]i in individual cells may be partly responsible for the different time courses of tension development of vascular smooth muscle in response to the vasoactive stimulants. The increase in [Ca~(2+)]i induced by ET-1 was transient but a pronounced and sustained contraction developed further in response to ET-1. Thus ET-1 has a biological property as a potential candidate to elicit cerebral vasospasm. Confocal laser microscopy could be a useful tool to measure the changes in [Ca~(2+)]i in individual smooth muscle cells of cerebral artery.
机译：平滑肌细胞内钙离子浓度（[Ca〜（2 +）] i）的变化在调节血管平滑肌张力和脑血管痉挛的发病机制中起着关键作用。在这项研究中，我们采用共聚焦激光显微镜来检测犬基底动脉单个平滑肌细胞产生的荧光信号。制备了环制剂，并装载了fluo-3，并通过共聚焦激光显微镜测量了由高钾离子和内皮素-1（ET-1）诱导的荧光变化。在一些未刺激的平滑肌细胞中，由细胞离散区域产生的Ca〜（2+）波以大约10＃mu＃m / s的速度传播到整个细胞。高K〜+（80 mmol / L）引起[Ca〜（2 +）] i快速升高，在刺激后约10 s一直达到峰值。相反，ET-1（0.3＃mu＃mol / L）诱导的[Ca〜（2 +）] i达到峰值水平的时间在单个细胞之间的13到26 s之间变化很大，这表明不一致的程度单个细胞中[Ca〜（2 +）] i增加的协调可能部分负责响应血管活性兴奋剂的血管平滑肌张力发展的不同时间过程。 ET-1引起的[Ca〜（2 +）] i的增加是短暂的，但对ET-1的响应却进一步产生了明显的持续收缩。因此，ET-1具有作为引发脑血管痉挛的潜在候选物的生物学特性。共聚焦激光显微镜可能是一种有用的工具，可以测量脑动脉单个平滑肌细胞中[Ca〜（2 +）] i的变化。

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