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Functional characterization of the 5 '-flanking and the promoter region of the human UCP3 (hUCP3) gene

机译：人UCP3（hUCP3）基因5'侧翼和启动子区域的功能表征

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Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5'-flanking region of the human UCP3 (hUCP3) gene. 5'-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a cl-acting negative regulatory element between -2983 and -982 while the region between -982 and -284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 23]
机译：解偶联蛋白3（UCP3）被认为是人类能量消耗和生热的重要调节剂。为了深入了解调节其表达的机制，我们已经克隆并鉴定了人UCP3（hUCP3）基因5'侧翼区域的约5 kb。 5'-RACE分析表明在翻译起始位点上游187bp的单个转录起始位点。启动子区域包含TATA和CAAT框，以及PPRE，TRE，CRE和肌肉特异性因子（如MyoD和MEF2位点）的共有基序。使用CAT-ELISA在多个细胞系中对3 kb hUCP3启动子片段进行功能鉴定，确定了-2983和-982之间的cl-作用负调控元件，而-982和-284之间的区域显示基础启动子活性大大增加，表明存在强的增强剂元素。在鼠骨骼肌细胞系C2C12中，启动子活性特别增强，反映了UCP3的组织选择性表达模式。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：23]

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