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首页> 外文期刊>Life sciences >Ovalbumin sensitization of guinea-pigs reduces fMLP-induced calcium signal in alveolar macrophages.
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Ovalbumin sensitization of guinea-pigs reduces fMLP-induced calcium signal in alveolar macrophages.

机译：豚鼠的卵清蛋白致敏作用降低了肺泡巨噬细胞中fMLP诱导的钙信号。

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摘要

In this study we analyzed the N-formyl-Met-Leu-Phe (fMLP)-induced calcium signal in alveolar macrophages (AM) isolated from ovalbumin-sensitized (OA-sensitized AM) and naive (naive AM) guinea-pigs. Guinea-pigs were sensitized by subcutaneous injection of OA and AM were isolated by bronchoalveolar lavage 6 weeks thereafter. On the following day, we measured in resting and fMLP-stimulated cells: intracellular calcium concentration by fura-2 imaging analysis, forskolin-induced cyclic AMP production and superoxide dismutase inhibitable superoxide anion release of adherent AM. Resting calcium was 82+/-5.0 nM (n=217) and 144+/-9.3 nM (n=213, P<0.001) in naive and OA-sensitized AM respectively. fMLP (10(-11)-10(-7)M) induced a dose-dependent calcium increase, 10(-8)M being the maximal effective dose in both naive and OA-sensitized AM. However, at all doses tested, this fMLP effect was lower in OA-sensitized than in naive AM. While in resting condition 10(-5)M forskolin increased cyclic AMP both in naive and OA-sensitized AM, in fMPL-stimulated AM forskolin was effective only in OA-sensitized AM. Superoxide anion release measured 10 min after fMLP stimulus was higher in naive than in sensitized AM. These data suggest that the fMLP-induced intracellular signal is different in OA-sensitized AM compared to naive cells.
机译：在这项研究中，我们分析了从卵清蛋白致敏（OA致敏AM）和天真（天真AM）豚鼠中分离出的肺泡巨噬细胞（AM）中N-甲酰基-Met-Leu-Phe（fMLP）诱导的钙信号。皮下注射OA使豚鼠致敏，并在6周后通过支气管肺泡灌洗分离AM。第二天，我们测量了静息和经fMLP刺激的细胞：通过fura-2成像分析的细胞内钙浓度，福司可林诱导的环AMP生成以及粘附AM的超氧化物歧化酶抑制性超氧化物阴离子释放。幼稚和OA致敏AM中的静息钙分别为82 +/- 5.0 nM（n = 217）和144 +/- 9.3 nM（n = 213，P <0.001）。 fMLP（10（-11）-10（-7）M）引起剂量依赖性钙增加，在幼稚和OA致敏AM中，10（-8）M是最大有效剂量。但是，在所有测试剂量下，OA致敏的fMLP效果均比幼稚AM低。在静止状态下，10（-5）M佛司可林在幼稚和OA致敏AM中均增加了环AMP，而在fMPL刺激的AM中佛司可林仅对OA致敏AM有效。 fMLP刺激后10分钟测得的超氧阴离子释放在幼稚期高于敏化AM。这些数据表明，与幼稚细胞相比，在OA致敏的AM中，fMLP诱导的细胞内信号有所不同。

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