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首页> 外文期刊>Cell and Tissue Research >Immunohistochemical localisation of pre-synaptic muscarinic receptor subtype-2 (M2r) in the enteric nervous system of guinea-pig ileum.
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Immunohistochemical localisation of pre-synaptic muscarinic receptor subtype-2 (M2r) in the enteric nervous system of guinea-pig ileum.

机译：豚鼠回肠肠神经系统中突触前毒蕈碱受体亚型2（M2r）的免疫组织化学定位。

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摘要

The cholinergic muscarinic 2 receptor (M2r) is known to be present on smooth muscle cells in the intestine. Pharmacological studies also suggest that M2rs regulate transmitter release from nerves in the enteric nervous system. This study localised M2rs in the guinea-pig ileum using different antibodies and fluorescence immunohistochemistry. Double labelling with antibodies against neurochemical markers was used to identify the type of nerves bearing M2r. Guinea-pig ileum were fixed, prepared for sections and wholemounts and incubated with antisera against the M2r sequence. Tissue was double labelled with antibodies against neuronal nitric oxide synthase (nNOS), common choline acetyltransferase (cChAT), substance P (SP), synaptophysin and vesicular acetylcholine transporter (VAChT). Immunofluorescence was viewed using confocal microscopy. Abundant M2r-immunoreactivity (IR) was present on the surface of circular and longitudinal smooth muscle cells. M2r-IR was present in many but not all nerve fibres in the circular muscle and ganglia. M2r-IR was present in VAChT-IR and cChAT-IR cholinergic nerve fibres and SP-IR nerve fibres in the myenteric ganglia and submucosal ganglia. M2r-IR was present on a few nNOS-IR nerve fibres and around nNOS-IR neurons in the myenteric ganglia. In the circular muscle and deep muscular plexus, M2r-IR was present in many VAChT-IR and SP-IR nerve fibres and in few nNOS-IR nerves. M2rs are not only present on muscle cells in the intestine, but also on nerve fibres. M2rs may mediate cholinergic reflexes via their location on muscle and also via neural transmission. The pre-synaptic location supports pharmacological studies suggesting M2rs mediate neurotransmitter release from nerve fibres. The presence of M2rs on VAChT-IR, SP-IR and nNOS-IR-containing nerve fibres suggests M2rs may regulate ACh, SP and nitric oxide release.
机译：已知胆碱能毒蕈碱2受体（M2r）存在于肠的平滑肌细胞上。药理研究还表明，M2rs调节肠道神经系统中神经的递质释放。这项研究使用不同的抗体和荧光免疫组织化学方法将M2rs定位于豚鼠回肠。使用针对神经化学标记物的抗体的双重标记来鉴定带有M2r的神经的类型。固定豚鼠回肠，准备切片和整装，并与针对M2r序列的抗血清一起孵育。组织用抗神经元一氧化氮合酶（nNOS），普通胆碱乙酰基转移酶（cChAT），P物质（SP），突触素和囊泡乙酰胆碱转运蛋白（VAChT）的抗体双重标记。使用共聚焦显微镜观察免疫荧光。圆形和纵向平滑肌细胞表面存在丰富的M2r免疫反应性（IR）。 M2r-IR存在于环形肌肉和神经节的许多但并非全部神经纤维中。 M2r-IR存在于肌间神经节和粘膜下神经节的VAChT-IR和cChAT-IR胆碱能神经纤维和SP-IR神经纤维中。 M2r-IR存在于肌层神经节的一些nNOS-IR神经纤维和nNOS-IR神经元周围。在VAChT-IR和SP-IR神经纤维和少数nNOS-IR神经中，M2r-IR存在于环形肌和深肌丛中。 M2rs不仅存在于肠中的肌肉细胞上，而且还存在于神经纤维上。 M2rs可能通过其在肌肉上的位置以及通过神经传递来介导胆碱能反射。突触前的位置支持药理研究，表明M2rs介导神经纤维释放神经递质。含有VAChT-IR，SP-IR和nNOS-IR的神经纤维上存在M2rs，这表明M2rs可能调节ACh，SP和一氧化氮的释放。

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