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首页> 外文期刊>Life sciences >Inhibitory effects of total flavones of Hippophae Rhamnoides L on thrombosis in mouse femoral artery and in vitro platelet aggregation.
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Inhibitory effects of total flavones of Hippophae Rhamnoides L on thrombosis in mouse femoral artery and in vitro platelet aggregation.

机译：沙棘总黄酮对小鼠股动脉血栓形成和体外血小板聚集的抑制作用。

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Total flavones of Hippophae Rhamnoides L (TFH) are extracted from Sea buckthorn, a Chinese herbal medicine. Sea buckthorn has antioxidant, anti-ulcerogenic and hepato-protective actions, and its berry oil is reported to suppress platelet aggregation. Though it is frequently used for patients with thrombosis, the likely mechanism(s) and effects of TFH on thrombogenesis remain unclear. Thus, we have investigated the effect in-vivo of TFH on thrombogenesis and in vitro on platelet aggregation, comparing them to those of aspirin.We measured thrombotic occlusion time in a mouse femoral artery thrombosis model by the photochemical reaction between intravenously injected rose bengal and green light irradiation. In vitro platelet aggregation in whole blood was measured by single platelet counting. Thrombotic occlusion time was 8.5 +/- 0.6 min in the control group. TFH at a dose of 300 micro g/kg, intravenously administered 15 min before the rose bengal injection, significantly prolonged it to 11.6 +/- 1.0 min (P < 0.05), a similar effect on in-vivo thrombogenesis to that of aspirin. TFH at a concentration of 3.0 micro g/ml significantly (P < 0.01) inhibited in vitro platelet aggregation induced by collagen (2 micro g/ml) in a concentration dependent manner, in contrast TFH did not affect aggregation induced by arachidonic acid (80 micro M) and ADP (0.3 micro M).The results of the present study, in which TFH prevented in-vivo thrombogenesis, probably due to inhibition of platelet aggregation, suggest a possible clinical approach for the prevention of thrombosis.
机译：沙棘总黄酮（TFH）是从沙棘（一种中草药）中提取的。沙棘具有抗氧化，抗溃疡和保肝作用，据报道其浆果油可抑制血小板聚集。尽管它经常用于血栓形成患者，但尚不清楚TFH对血栓形成的可能机制和作用。因此，我们研究了TFH体内对血栓形成的影响以及体外对血小板聚集的影响，并与阿司匹林进行了比较。绿光照射。通过单次血小板计数来测量全血中的体外血小板聚集。对照组血栓闭塞时间为8.5 +/- 0.6分钟。玫瑰红注射前15分钟静脉给予300微克/千克的TFH，将其显着延长至11.6 +/- 1.0分钟（P <0.05），对体内血栓形成的作用与阿司匹林相似。浓度为3.0 micro g / ml的TFH（P <0.01）以浓度依赖性方式显着抑制胶原（2 micro g / ml）诱导的体外血小板凝集，相反TFH则不影响花生四烯酸（80 micro M）和ADP（0.3 micro M）。本研究的结果（其中TFH可能是由于抑制了血小板凝集而阻止了体内血栓形成）提示了预防血栓形成的可能临床方法。

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