• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Stretch-induced morphological changes of human endothelial cells depend on the intracellular level of Ca2+ rather than of cAMP.
【24h】

Stretch-induced morphological changes of human endothelial cells depend on the intracellular level of Ca2+ rather than of cAMP.

机译：拉伸诱导的人内皮细胞形态变化取决于细胞内Ca2 +水平而不是cAMP。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

When exposed to a uni-axial cyclic stretch, cultured human umbilical vein endothelial cells (HUVECs) align and elongate perpendicular to the stretch axis. Previous studies showed that forskolin inhibited stretch-induced orientation of endothelial cells, suggesting that adenosine 3:5-cyclic monophosphate (cAMP) plays an important role in the shape change. However, we have recently shown that stretch-induced shape changes in cultured HUVECs are due to increased [Ca2+]i. In the present study, we examined the possible role of cAMP in stretch-induced shape changes in cultured HUVECs. Application of uni-axial cyclic stretch induced a gradual rise in cAMP reaching a peak level at 60 min after the onset of stretch. The adenylate cyclase activator, forskolin, increased the basal level of cAMP but inhibited the rise in [Ca2+]i resulting in no cell shape changes. In contrast, N 6,2-dibutyryladenosine 3:5-cyclic monophosphate (dbcAMP) enhanced the stretch-induced increase in cAMP and [Ca2+]i and resulted in cell shape changes. On the other hand, 2'5'-dideoxyadenosine (DDA), an adenylate cyclase inhibitor, inhibited stretch-induced increases in cAMP and [Ca2+]i resulting in no cell shape changes. In summary, our data showed that cell shape changes were consistently dependent on [Ca2+]i rather than cAMP levels. We conclude that the primary second messenger in the stretch-induced shape changes in HUVECs is intracellular Ca2+ rather than cAMP.
机译：当暴露于单轴循环拉伸时，培养的人脐静脉内皮细胞（HUVEC）排列并垂直于拉伸轴伸长。先前的研究表明，福司可林抑制拉伸诱导的内皮细胞定向，表明腺苷3：5-环一磷酸（cAMP）在形状改变中起重要作用。但是，我们最近显示，在培养的HUVEC中，拉伸诱导的形状变化是由于[Ca2 +] i增加所致。在本研究中，我们研究了cAMP在培养的HUVEC拉伸诱导的形状变化中的可能作用。单轴循环拉伸的应用导致cAMP逐渐升高，在拉伸开始后60分钟达到峰值。腺苷酸环化酶激活剂福司可林增加了cAMP的基础水平，但抑制了[Ca2 +] i的升高，导致细胞形状没有变化。相反，N 6,2-二丁酰腺苷3：5-环一磷酸（dbcAMP）增强了拉伸诱导的cAMP和[Ca2 +] i的增加，并导致细胞形状改变。另一方面，腺苷酸环化酶抑制剂2'5'-二脱氧腺苷（DDA）抑制了拉伸诱导的cAMP和[Ca2 +] i的增加，导致细胞形状没有变化。总而言之，我们的数据表明细胞形状的变化始终取决于[Ca2 +] i，而不是cAMP水平。我们得出结论，HUVEC中拉伸诱导的形状变化中的主要第二信使是细胞内Ca2 +，而不是cAMP。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号