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首页> 外文期刊>Life sciences >Differential expression of AP-2gamma and AP-2alpha during human trophoblast differentiation.
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Differential expression of AP-2gamma and AP-2alpha during human trophoblast differentiation.

机译：人类滋养细胞分化过程中AP-2γ和AP-2alpha的差异表达。

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摘要

Previous studies from our laboratory demonstrated that AP-2alpha induces the expression of the hPL and hCG genes in cultured trophoblast cells. In the current study, we have shown by transient transfection studies that AP-2gamma, which is the product of a separate gene from AP-2alpha, also stimulates hPL and hCGbeta promoter activities. However, AP-2gamma mRNA levels during in vitro differentiation of human cytotrophoblast cells were strikingly different than those of AP-2alpha mRNA levels, with AP-2alpha increasing and AP-2gamma markedly decreasing during the differentiation process. The amount of AP-2gamma protein binding to AP-2 elements on the hPL promoter, as determined by supershift assays, also markedly decreased during the differentiation process. These findings strongly suggest that AP-2gamma action in cytotrophoblast cells is repressed by a co-factor(s) that inhibits AP-2gamma action or is prevented by the absence of a co-factor(s) that is essential for AP-2gamma action.
机译：我们实验室先前的研究表明，AP-2alpha诱导培养的滋养细胞中hPL和hCG基因的表达。在当前的研究中，我们通过瞬时转染研究表明，AP-2γ是一种与AP-2alpha分离的基因，它还刺激hPL和hCGbeta启动子活性。然而，人类成纤维细胞体外分化过程中的AP-2gamma mRNA水平与AP-2alpha mRNA的水平显着不同，在分化过程中AP-2alpha升高而AP-2gamma明显降低。通过超移位测定法测定，与hPL启动子上的AP-2元素结合的AP-2gamma蛋白的量在分化过程中也明显减少。这些发现强烈表明，成纤维细胞中AP-2γ的作用受到抑制AP-2γ作用的辅因子的抑制，或者由于缺乏对AP-2γ作用必不可少的辅因子而被阻止。

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