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首页> 外文期刊>Life sciences >The localization of dopamine D2 receptor mRNA in the human placenta and the anti-angiogenic effect of apomorphine in the chorioallantoic membrane.
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The localization of dopamine D2 receptor mRNA in the human placenta and the anti-angiogenic effect of apomorphine in the chorioallantoic membrane.

机译：多巴胺D2受体mRNA在人胎盘中的定位以及阿扑吗啡在绒毛膜尿囊膜中的抗血管生成作用。

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摘要

While there are some reports indicating that dopamine (DA) and D2-like receptors (Rc) are detected in human placenta, there is little evidence available regarding the function of DA or the precise localization of its receptors in this organ. In the present study, we confirmed the placental expression of DA D2 Rc transcripts by Northern blot analysis. Using in situ hybridization, we also first revealed that DA D2 Rc mRNA was expressed in cytotrophoblasts, syncytial trophoblasts, vascular endothelial cells, Hafbauer cells, and fibroblasts in the chorionic villi of the human placenta. The expression sites of DA D2 Rc mRNA led us to suspect other functions of DA in the placenta besides the regulation of human placental lactogen. Since the cells expressing DA D2 Rc mRNA are related to proliferation and remodeling of placental tissue, we tried to evaluate a possible involvement of DA in the regulation of placental angiogenesis. To this end, we used the chicken chorioallantoic membrane (CAM) assay. In CAM assay, apomorphine, a potent nonselective agonist of DA, has an anti-angiogenic effect. These results suggest that DA may regulate the vascularization of human placenta through its receptors present in the chorionic villi.
机译：尽管有一些报道表明在人胎盘中检测到了多巴胺（DA）和D2样受体（Rc），但关于DA的功能或其受体在该器官中的精确定位的证据很少。在本研究中，我们通过Northern印迹分析证实了DA D2 Rc转录本的胎盘表达。使用原位杂交，我们还首先揭示了DA D2 Rc mRNA在人胎盘绒毛膜绒毛细胞的滋养细胞，合胞体滋养细胞，血管内皮细胞，Hafbauer细胞和成纤维细胞中表达。 DA D2 Rc mRNA的表达位点使我们怀疑除了人胎盘乳原的调控外，DA在胎盘中的其他功能。由于表达DA D2 Rc mRNA的细胞与胎盘组织的增殖和重塑有关，因此我们试图评估DA在胎盘血管生成调控中的可能参与。为此，我们使用了鸡绒膜尿囊膜（CAM）分析。在CAM分析中，阿扑吗啡（一种有效的DA非选择性激动剂）具有抗血管生成作用。这些结果表明，DA可以通过绒毛膜绒毛中存在的受体来调节人胎盘的血管形成。

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