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首页> 外文期刊>Life sciences >GLIS, a bioactive proteoglycan fraction from Ganoderma lucidum, displays anti-tumour activity by increasing both humoral and cellular immune response.
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GLIS, a bioactive proteoglycan fraction from Ganoderma lucidum, displays anti-tumour activity by increasing both humoral and cellular immune response.

机译：GLIS是灵芝的一种生物活性蛋白聚糖级分，通过增加体液和细胞免疫应答而显示出抗肿瘤活性。

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摘要

AIMS: Ganoderma lucidum, a traditional Chinese medicine, is well known as a modulator of functions of the immune system as well as an anti-tumour agent. However, its active compounds and their molecular mechanisms of action are not well established. GLIS, a proteoglycan isolated from the fruiting body of G. lucidum, stimulates directly the activation of B lymphocytes. In this work, the immunoactivation capacities of GLIS as well as its anti-tumour effect were investigated in vitro and in vivo. MAIN METHODS: Tumour-bearing mice were prepared by inoculation of mouse sarcoma S180 cells into BALB/c mice. Lymphocytes and bone marrow-derived macrophages were isolated from spleen and tibia/femurs, respectively. After stimulation with GLIS different immune responses of these cells were analysed. Anti-tumour effect of GLIS was determined. KEY FINDINGS: After treatment with GLIS, spleen-derived B lymphocytes from tumour-bearing mice became activated, proliferated and produced large amounts of immunoglobulins. Bone marrow-derived macrophages from tumour-bearing mice also became activated after exposure to GLIS, and they produced important immunomodulatory substances, such as IL-1beta, TNF-alpha and reactive nitrogen intermediates, like NO. GLIS markedly increased phagocytosis of macrophages, and very importantly, it markedly raised the macrophage-mediated tumour cytotoxicity. Treatment of mice with GLIS caused an inhibition of mouse sarcoma S180 tumour growth by 60% in vivo. SIGNIFICANCE: These results indicate that GLIS exhibits a capacity to increase remarkably both humoral and cellular immune activities of tumour-bearing mice and inhibits tumour growth significantly. The anti-tumour effect of GLIS results from its capacity to increase the host's immune activity.
机译：目的：灵芝是一种传统中药，众所周知是免疫系统功能的调节剂和抗肿瘤剂。然而，其活性化合物及其作用的分子机理尚不十分清楚。 GLIS是从灵芝子实体中分离出来的一种蛋白聚糖，直接刺激B淋巴细胞的激活。在这项工作中，在体外和体内研究了GLIS的免疫激活能力及其抗肿瘤作用。主要方法：通过将小鼠肉瘤S180细胞接种到BALB / c小鼠中来制备荷瘤小鼠。分别从脾脏和胫骨/股骨中分离出淋巴细胞和骨髓源性巨噬细胞。用GLIS刺激后，分析了这些细胞的不同免疫应答。确定了GLIS的抗肿瘤作用。主要发现：GLIS治疗后，来自荷瘤小鼠的脾脏B淋巴细胞被激活，增殖并产生大量免疫球蛋白。荷瘤小鼠的骨髓衍生巨噬细胞在暴露于GLIS后也被激活，它们产生重要的免疫调节物质，例如IL-1beta，TNF-α和活性氮中间体，如NO。 GLIS明显增加了巨噬细胞的吞噬作用，而且非常重要的是，它显着提高了巨噬细胞介导的肿瘤细胞毒性。用GLIS治疗小鼠在体内引起小鼠肉瘤S180肿瘤生长的抑制60％。意义：这些结果表明，GLIS能够显着提高荷瘤小鼠的体液和细胞免疫活性，并显着抑制肿瘤生长。 GLIS的抗肿瘤作用来自其增强宿主免疫活性的能力。

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