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首页> 外文期刊>Cell and Tissue Research >Visualization of structural organization of ventral membranes of sheared-open resorbing osteoclasts attached to apatite pellets
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Visualization of structural organization of ventral membranes of sheared-open resorbing osteoclasts attached to apatite pellets

机译：附着在磷灰石颗粒上的剪切开放吸收破骨细胞腹膜结构组织的可视化

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摘要

Osteoclasts are highly polarized cells from both morphological and functional points of view. Using quick-freeze, rotary-replication methods combined with cell-shearing, we clarified the variability of cytoplasmic surface of the polarized membranes of osteoclasts seeded on apatite. As to the organization of actin filaments and clathrin sheets, we confirmed almost the same ventral membrane specializations of osteoclasts on apatite as seen on glass plates. The organized actin filaments and membrane-associated particles supported the ruffled border membranes. Inside the actin sealing zone, membrane specializations were not always occupied with the ruffled border but also with other types of membranes. Some osteoclasts formed an actin ring but lacked the ruffled border projections. We report a unique and distinctive membrane modification of apatite-attached osteoclasts, i.e., the presence of dense aggregates of membrane-associated particles and related structures not found in the osteoclasts seeded on glass plates. Actin filament polarity in the podosomes was determined by decoration with myosin S1. The actin filament polarity within podosome appears to be oriented predominantly with its barbed ends toward the core, whereas the interconnecting F-actin appears to be mixed oriented. Two different types of clathrin plaques displayed different distributions: clathrin-dependent endocytosis was observed in the ruffled border regions, whereas flat clathrin sheets were found in the leading edge of lamellipodia and near podosomes. The clathrin sheets adhered to the apatite surface tightly on the ventral membranes overlaying the resorption lacunae. All these membrane specializations as mentioned above may indicate the functional variability of osteoclasts seeded on apatite.
机译：从形态和功能的角度来看，破骨细胞都是高度极化的细胞。使用速冻，旋转复制方法与细胞剪切相结合，我们阐明了磷灰石播种的破骨细胞极化膜细胞质表面的变异性。至于肌动蛋白丝和网格蛋白片的组织，我们证实了磷灰石上破骨细胞腹膜的特化几乎与玻璃板上的相同。有组织的肌动蛋白丝和与膜相关的颗粒支持皱纹的边界膜。在肌动蛋白密封区域内，褶皱的边界并不总是占据着膜专业领域，而其他类型的膜也总是占据着优势。一些破骨细胞形成肌动蛋白环，但缺乏皱纹的边界突起。我们报道了附有磷灰石的破骨细胞的独特和独特的膜修饰，即存在在玻璃板上接种的破骨细胞中找不到的膜相关颗粒和相关结构的密集聚集体。通过用肌球蛋白S1修饰确定足小体中的肌动蛋白丝极性。足囊内的肌动蛋白丝极性似乎主要以其带刺的末端朝向核心取向，而相互连接的F-肌动蛋白似乎是混合取向的。两种不同类型的网格蛋白斑块表现出不同的分布：在褶皱的边缘区域观察到网格蛋白依赖性内吞作用，而在lamellipodia的前缘和足小体附近发现了平坦的网格蛋白片。网格蛋白片紧密地粘附在磷灰石表面的腹膜上，覆盖了吸收腔。如上所述，所有这些膜特化可能表明播种在磷灰石上的破骨细胞的功能变异性。

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