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首页> 外文期刊>Cell and Tissue Research >Cytokine production suppression by culture supernatant of B16F10 cells and amelioration by Ganoderma lucidum polysaccharides in activated lymphocytes
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Cytokine production suppression by culture supernatant of B16F10 cells and amelioration by Ganoderma lucidum polysaccharides in activated lymphocytes

机译：B16F10细胞培养上清液对细胞因子产生的抑制作用以及灵芝多糖对活化淋巴细胞的抑制作用

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摘要

Some cytokines, such as interleukin-2 (IL-2), interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha), produced by lymphocytes might play an important role in anti-tumor immunity and their production is possibly suppressed by cancer. Amelioration of the suppression of cytokine production might contribute to cancer control. Ganoderma lucidum polysaccharides (Gl-PS), a versatile group of a component of G. lucidum and one with various bioactivities, might have this potential. In this study, analyses including reverse transcription and the polymerase chain reaction (RT-PCR), immunocytochemistry and Western blot were used to test the effects of Gl-PS on the production of IL-2, IFN-gamma and TNF-alpha in mononuclear lymphocytes by incubating Gl-PS with mouse splenic mononuclear lymphocytes in the presence of B16F10 cell culture supernatant following activation by phytohemagglutinin. The RT-PCR, immunocytochemistry and Western blot assays showed that the production of IL-2, IFN-gamma and TNF-alpha in mononuclear lymphocytes was suppressed by B16F10 cell culture supernatant at both the mRNA and protein levels, whereas the suppression was fully or partially ameliorated by Gl-PS. The amelioration by Gl-PS against the suppression of the production of IL2, IFN-gamma and TNF-alpha in mononuclear lymphocytes by B16F10 cell culture supernatant might contribute to cancer control.
机译：淋巴细胞产生的某些细胞因子，例如白介素2（IL-2），干扰素-γ（IFN-γ）和肿瘤坏死因子-α（TNF-α），可能在抗肿瘤免疫及其产生中起重要作用可能被癌症抑制了。改善细胞因子产生的抑制作用可能有助于控制癌症。灵芝多糖（G1-PS）是灵芝成分的通用组，具有多种生物活性，可能具有这种潜力。在这项研究中，使用了包括逆转录和聚合酶链反应（RT-PCR），免疫细胞化学和蛋白质印迹在内的分析来测试G1-PS对单核细胞IL-2，IFN-γ和TNF-α产生的影响。在通过植物血凝素激活后，在B16F10细胞培养上清液的存在下，通过将G1-PS与小鼠脾脏单核淋巴细胞一起温育来获得淋巴细胞。 RT-PCR，免疫细胞化学和Western blot分析显示，B16F10细胞培养上清液在mRNA和蛋白水平上均抑制了单核淋巴细胞中IL-2，IFN-γ和TNF-α的产生，而这种抑制作用被完全或完全抑制。 Gl-PS可以部分改善。 B16F10细胞培养上清液对G1-PS抑制单核淋巴细胞IL2，IFN-γ和TNF-α产生的抑制作用可能有助于癌症控制。

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