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首页> 外文期刊>Life sciences >A membrane protein associated with the prolactin receptor. Studies with a photoactivatable human growth hormone derivative.
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A membrane protein associated with the prolactin receptor. Studies with a photoactivatable human growth hormone derivative.

机译：与催乳激素受体相关的膜蛋白。用可光活化的人类生长激素衍生物进行研究。

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摘要

Prolactin receptor from rat liver (PRL-R, 42 kDa) was cross-linked to a radiolabeled azidophenacyl derivative of human growth hormone ([125I]AP-hGH) to yield a 63 kDa adduct. In addition, a protein of Mr 50-52 K was detected as a 73 kDa complex. Microsomes incubated with either (a) increasing amounts of [125I]AP-hGH, or (b) a fixed amount of photoprobe and increasing concentrations of unlabeled hGH, showed that the 73/63 kDa band intensity ratio remains constant (0.71-0.77). Once transferred onto nitrocellulose membranes, only the 42 kDa protein is able to bind [125I]AP-hGH or [125I]hGH. Two anti-PRL-R monoclonal antibodies fail to cross-react with proteins of Mr 50-52 K. In membranes solubilized with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS), a significantly lower amount of the 73 kDa complex is detected. Thus, the 50-52 kDa protein appears to be structurally unrelated to, but is presumably associated with the PRL-R. The 73 kDa complex is also detected under low membrane fluidity conditions (1 degree C), indicating that PRL-R associates to this 50-52 kDa protein prior to hormone binding. Perfusion of rat liver with [125I]AP-hGH shows that this associated protein accompanies the receptor along its intracellular pathway.
机译：将来自大鼠肝脏的催乳激素受体（PRL-R，42 kDa）与人生长激素（[125I] AP-hGH）的放射性标记的叠氮苯甲酰基衍生物交联，得到63 kDa的加合物。另外，检测到Mr 50-52 K的蛋白质为73 kDa复合物。与（a）增加量的[125I] AP-hGH或（b）固定量的光探针和增加浓度的未标记hGH一起孵育的微粒体显示73/63 kDa谱带强度比保持恒定（0.71-0.77） 。一旦转移到硝酸纤维素膜上，只有42 kDa蛋白能够结合[125I] AP-hGH或[125I] hGH。两种抗PRL-R单克隆抗体无法与Mr 50-52 K的蛋白质发生交叉反应。在被3-[（（3-胆酰胺基丙基）-二甲基铵] -1-丙磺酸盐（CHAPS）溶解的膜中，检测到73 kDa复合物。因此，50-52 kDa蛋白似乎在结构上与PRL-R不相关，但可能与PRL-R相关。在低膜流动性条件下（1摄氏度）也检测到73 kDa的复合物，表明PRL-R在激素结合之前先与该50-52 kDa的蛋白质缔合。用[125I] AP-hGH向大鼠肝脏灌注显示该相关蛋白沿其细胞内途径伴随着受体。

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