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首页> 外文期刊>Life sciences >L-GLUTAMATE-INDUCED CHANGES IN INTRACELLULAR CALCIUM OSCILLATION FREQUENCY THROUGH NON-CLASSICAL GLUTAMATE RECEPTOR BINDING IN CULTURED RAT MYOCARDIAL CELLS
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L-GLUTAMATE-INDUCED CHANGES IN INTRACELLULAR CALCIUM OSCILLATION FREQUENCY THROUGH NON-CLASSICAL GLUTAMATE RECEPTOR BINDING IN CULTURED RAT MYOCARDIAL CELLS

机译：L-谷氨酸诱导的非经典谷氨酸受体结合在培养大鼠心肌细胞中的钙内振荡频率的变化

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The effects of L-glutamate, N-methyl-D-aspartate (NMDA), kainate (KA) and L-alpha-amino-3-hydroxy-5-methyl-4-isoxazole proprionate (AMPA) on intracellular Ca2+ oscillation frequency were studied in cultured rat myocardial cells. Ca2+ oscillations per minute were increased as compared to control by L-glutamate (100 mu M) from 3.8 +/- 2.2 to 25.7 +/- 4.3 (p < 0.001) and the NMDA-receptor agonist, NMDA (100 mu M), from 1.2 +/- 0.8 to 34.8 +/- 10.1 (p < 0.011). Increases over control frequency were also seen in response to the non-NMDA receptor agonists KA (100 mu M) from 5.8 +/- 2.3 to 25.6 +/- 3.2 (p < 0.001) and AMPA (10 mu M) from 3.8 +/- 1.2 to 13.3 +/- 1.8 (p < 0.001). The non-competitive NMDA receptor antagonist, (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK801) (10 mu M), decreased the Ca2+ oscillation frequency induced by NMDA (100 mu M from 36.8 +/- 12.2 to 7.2 +/- 7.2 (p < 0.05). (+/-)-2-Amino-7-phosphonoheptanoic acid (AP7), a competitive inhibitor at the NMDA receptor inhibited the increase in frequency induced by KA (100 mu M) at all concentrations tested (0.1, 1.0, 10 and 100 mu M). 6,7-Dinitroquinoxaline-2,3-dione (DNQX), a competitive inhibitor at non-NMDA receptors, also decreased the oscillation frequency elicited by KA (100 mu M) from 35.4 +/- 9.4 to 28.2 +/- 9.8, 24.8 +/- 9.8 and 11 +/- 9.5 at concentrations of 0.1, 1.0 and 10 mu M respectively. The peak amount of intracellular Ca2+ as expressed as the fluo 3 ratio, F/F-rest, was not increased by L-glutamate, NMDA or KA. These results suggest the presence of a novel glutamate receptor composed of both non-NMDA and NMDA subunits on cultured rat myocardial cells, and receptor stimulation leads to an increase in intracellular Ca2+ oscillation frequency. [References: 38]
机译：L-谷氨酸，N-甲基-D-天冬氨酸（NMDA），海藻酸酯（KA）和L-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸酯（AMPA）对细胞内Ca2 +振荡频率的影响是在培养的大鼠心肌细胞中进行了研究。与L-谷氨酸（100μM）相比，每分钟Ca2 +振荡增加，从3.8 +/- 2.2增至25.7 +/- 4.3（p <0.001）和NMDA受体激动剂NMDA（100μM），从1.2 +/- 0.8到34.8 +/- 10.1（p <0.011）。非NMDA受体激动剂KA（100μM）从5.8 +/- 2.3增至25.6 +/- 3.2（p <0.001）和AMPA（10μM）从3.8 + /起，控制频率也有所增加-1.2至13.3 +/- 1.8（p <0.001）。非竞争性NMDA受体拮抗剂（+）-5-甲基-10,11-二氢-5H-二苯并[a，d]环庚-5,10-亚胺马来酸酯（MK801）（10μM）降低了Ca2 + NMDA（100μM从36.8 +/- 12.2到7.2 +/- 7.2（p <0.05）引起的振荡频率。（+/-）-2-Amino-7-phosphonoheptanoic acid（AP7），一种竞争性抑制剂在所有测试浓度（0.1、1.0、10和100μM）下，NMDA受体均抑制KA（100μM）诱导的频率增加，竞争性抑制剂6,7-Dinitroquinoxaline-2,3-dione（DNQX）在非NMDA受体也可以将KA（100μM）引起的振荡频率从35.4 +/- 9.4降低到28.2 +/- 9.8、24.8 +/- 9.8和11 +/- 9.5（浓度分别为0.1、1.0和10） L-谷氨酸，NMDA或KA不会增加以fluo 3比F / F-rest表示的细胞内Ca2 +的峰值量，这些结果表明存在由两种非谷氨酸组成的新型谷氨酸受体-NMDA和NMDA亚基在培养的大鼠心肌上细胞，受体刺激导致细胞内Ca2 +振荡频率增加。 [参考：38]

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