• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Modified natural porcine surfactant modulates tobacco smoke-induced stress response in human monocytes.
【24h】

Modified natural porcine surfactant modulates tobacco smoke-induced stress response in human monocytes.

机译：改性天然猪表面活性剂可调节烟草烟雾诱导的人类单核细胞应激反应。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tobacco smoke (TS) is a potent source of oxidants and oxidative stress is an important mechanism by which TS exerts its toxicity in the lung. We have shown that TS induces heat shock (HS)/stress protein (HSP) synthesis in human monocytes. Pulmonary surfactant (PS) whose major physiological function is to confer mechanical stability to alveoli, also modulates oxidative metabolism and other pro-inflammatory functions of monocytes-macrophages. In order to determine whether PS alters the stress response induced by TS, we incubated human peripheral blood monocytes overnight with modified natural porcine surfactant (Curosurf) (1 mg/ml) before exposure to TS. Curosurf decreased TS-induced, but not HS-induced, expression of the major cytosolic, inducible 72 kD HSP (Hsp70). Furthermore, TS-generated superoxide anions production was significantly decreased by Curosurf in an acellular system, suggesting a direct scavenging effect of PS. We also examined the effects of TS and PS on monocytes ultrastructure. Monocytes incubated with Curosurf presented smoother cell membranes than control monocytes, while TS-induced monocyte vacuolization was, at least in part, prevented by Curosurf. Taken together, our data suggest that PS plays a protective role against oxygen radical-mediated, TS-induced cellular stress responses.
机译：烟草烟雾（TS）是氧化剂的有效来源，氧化应激是TS在肺部发挥毒性的重要机制。我们已经显示TS诱导人类单核细胞中的热休克（HS）/应激蛋白（HSP）合成。肺表面活性剂（PS），其主要生理功能是赋予肺泡机械稳定性，还调节单核细胞-巨噬细胞的氧化代谢和其他促炎功能。为了确定PS是否会改变TS诱导的应激反应，我们在暴露于TS之前，将人外周血单核细胞与修饰的天然猪表面活性剂（Curosurf）（1 mg / ml）孵育过夜。 Curosurf降低了TS诱导但不是HS诱导的主要胞质可诱导72 kD HSP（Hsp70）的表达。此外，Curosurf在无细胞系统中可显着降低TS产生的超氧阴离子的产生，表明PS具有直接清除作用。我们还检查了TS和PS对单核细胞超微结构的影响。 Curosurf孵育的单核细胞比对照单核细胞呈现出更光滑的细胞膜，而Curosurf至少部分阻止了TS诱导的单核细胞空泡化。两者合计，我们的数据表明PS对氧自由基介导的TS诱导的细胞应激反应起保护作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号