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首页> 外文期刊>Life sciences >Semiquantitative evaluation of alpha1A-adrenoceptor subtype mRNA in human hypertrophied and non-hypertrophied prostates: regional comparison.
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Semiquantitative evaluation of alpha1A-adrenoceptor subtype mRNA in human hypertrophied and non-hypertrophied prostates: regional comparison.

机译：在人类肥大和非肥大前列腺中的alpha1A-肾上腺素受体亚型mRNA的半定量评估：区域比较。

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摘要

The main purpose of this study was to compare semiquantitatively the amount of alpha1a-adrenoceptor subtype mRNA in urethral, central and peripheral areas in benign hypertrophied prostates and non-hypertrophied prostate by the competitive reverse transcriptase polymerase chain reaction (RT-PCR) method. Prostates from twenty cases of men with benign prostatic hypertrophy (BPH) and 5 cases of bladder tumor without BPH were obtained for this study. The mRNA was extracted from the urethral, central and peripheral areas of each prostate, and quantitative RT-PCR was performed using the ratio of PCR product of alpha1a-adrenoceptor mRNA/beta2-microglobulin mRNA. The ratio for the central area of the hypertrophied prostate was significantly greater than that for the same area of the non-hypertrophied prostate (p < 0.05). In contrast, the urethral area showed no significant difference between the two prostate conditions. The central area of the hypertrophied prostate showed the tendency to have an increased alpha1a-adrenoceptor mRNA level compared with the urethral area, though no statistical difference was recognized because of the high standard error. Regional differences in the alpha1a-adrenoceptor mRNA in the non-hypertrophied prostate were rarely observed. The present finding demonstrates that the increased alpha1a-adrenoceptor mRNA content found in the hypertrophied prostate (1) was due to that in the central area.
机译：这项研究的主要目的是通过竞争性逆转录酶聚合酶链反应（RT-PCR）方法半定量比较良性肥厚性前列腺和非肥大性前列腺的尿道，中枢和外周区域中α1a-肾上腺素受体亚型mRNA的量。本研究从20例男性前列腺增生（BPH）男性和5例无BPH膀胱肿瘤的男性中获取前列腺。从每个前列腺的尿道，中央和周边区域提取mRNA，并使用α1a-肾上腺素受体mRNA /β2-微球蛋白mRNA的PCR产物的比率进行定量RT-PCR。肥大的前列腺中央区域的比例明显大于非肥大的前列腺相同区域的比例（p <0.05）。相反，在两种前列腺疾病之间，尿道面积没有显示出显着差异。与尿道区域相比，肥大的前列腺中央区域显示出具有增加的α1a-肾上腺素受体mRNA水平的趋势，尽管由于高标准误而没有统计学差异。很少在非肥厚性前列腺中观察到α1a-肾上腺素受体mRNA的区域差异。本发现表明，在肥厚的前列腺中发现的α1a-肾上腺素受体mRNA含量增加（1）是由于在中央区域。

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