Preparation of highly purified momordin II without ribonuclease activity

Paola Valbonesi; Luigi Barbieri; Andrea Bolognesi; Elena Bonora; Letizia Polito; Fiorenzo Stirpe

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Preparation of highly purified momordin II without ribonuclease activity

机译：没有核糖核酸酶活性的高纯度莫莫丁II的制备

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Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribosomes and from DNA, and had no RNase activity.

机译：Momordin II是一种来自Momordica charantia种子的核糖体失活蛋白，可通过涉及在S-Sepharose，Sephadex G-50，CM-Sepharose和Red Sepharose色谱柱上进行一系列色谱分离的方法纯化。高度纯化的Momordin II抑制无细胞蛋白质合成，从大鼠肝核糖体和DNA中释放出腺嘌呤，并且没有RNase活性。

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《Life sciences》 |1999年第14期|共7页
作者
Paola Valbonesi; Luigi Barbieri; Andrea Bolognesi; Elena Bonora; Letizia Polito; Fiorenzo Stirpe;
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正文语种 eng
中图分类生命的起源;生命科学总论;
关键词
momordin; ribosome-inactivating proteins; polynucleotide: adenosine glycosidase; ribonuclease;

机译：苦瓜素;核糖体失活蛋白;多核苷酸：腺苷糖苷酶;核糖核酸酶;

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1. Preparation of highly purified momordin II without ribonuclease activity [J] . Paola Valbonesi, Luigi Barbieri, Andrea Bolognesi, Life sciences . 1999,第14期

机译：没有核糖核酸酶活性的高纯度莫莫丁II的制备
2. Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography [J] . Lu Lu, Liu Yanping, Ding Yue, Natural product research . 2016,第19a21期

机译：酶联法与制备型液相色谱法联用知母制备高纯度的皂草苷AIII
3. Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography [J] . Lu Lu, Liu Yanping, Ding Yue, Natural product research . 2016,第19a21期

机译：用酶法与制备液相色谱法用酶法制备高纯化的TIMOSAKONINAIII AIII
4. Separating of Productive and Highly Purified Mogrosides from Siraitia grosvenorii [C] . Liu Can, Liu Jing, Rong Yonghai, 5th International Conference on Bioinformatics and Biomedical Engineering . 2011

机译：从罗汉果（Siraitia grosvenorii）中分离生产性高纯度罗汉果甙
5. Effects of the ribonuclease inhibitor on the biological activity of pancreatic-type ribonucleases. [D] . Dickson, Kimberly Anne. 2006

机译：核糖核酸酶抑制剂对胰腺型核糖核酸酶生物学活性的影响。
6. Synapsin I (protein I) a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation [O] . 1983

机译：Synapsin I（蛋白I）一种神经末梢特异性磷蛋白。三在高度纯化的突触小泡制剂中研究了其与突触小泡的关联
7. FRACTIONATION OF PEPSIN : I. ISOLATION OF CRYSTALLINE PEPSIN OF CONSTANT ACTIVITY AND SOLUBILITY FROM PEPSINOGEN OR COMMERCIAL PEPSIN PREPARATIONS. II. PREPARATION OF A LESS SOLUBLE FRACTION. III. SOLUBILITY CURVES OF MIXTURES OF THE SOLUBLE AND INSOLUBLE FRACTIONS. IV. PREPARATION OF HIGHLY ACTIVE PEPSIN FROM PEPSINOGEN [O] . Herriott, Roger M., Desreux, V., Northrop, John H. 1940

机译：胃蛋白酶的分馏：I.从胃蛋白酶原或商业胃蛋白酶制剂中分离出具有恒定活性和可溶性的结晶胃蛋白酶。二。少量可溶级分的制备。三，可溶性和不溶级分的混合物的溶解度曲线。 IV。从胃蛋白酶原制备高活性胃蛋白酶

Preparation of highly purified momordin II without ribonuclease activity

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