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首页> 外文期刊>Life sciences >In situ model for studying potassium currents in various growth plate chondrocyte subpopulations
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In situ model for studying potassium currents in various growth plate chondrocyte subpopulations

机译：用于研究各种生长板软骨细胞亚群中钾电流的原位模型

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A new in situ model of partially digested growth plate cartilage suitable for patch clamp study of membrane currents of chondrocytes from various differentiation stages was developed. Thin sections of growth plate were enzyme digested to expose intact membranes of chondrocytes previously covered by extracellular matrix. This treatment dramatically increased the success rate of tight-seal formation from virtually 0% up to 40%. Whole-cell patch clamp recording revealed a delayed outward rectifying current as the major macroscopic current in chondrocytes of all differentiation stages. This current was sensitive to tetraethylammonium chloride and reversed polarity at a membrane potential close to the equilibrium potential of K+. Chondrocytes at resting stage expressed a much smaller K+ current than the proliferative and hypertrophic chondrocytes. When the current amplitudes were normalized for the cell membrane area, proliferative cells expressed a significantly higher outward current density. (C) 2001 Elsevier Science Inc. All rights reserved. [References: 18]
机译：建立了新的部分消化的生长板软骨原位模型，适用于膜片钳研究不同分化阶段软骨细胞膜电流。酶消化生长板的薄片以暴露先前被细胞外基质覆盖的软骨细胞的完整膜。这种治疗大大提高了紧密密封形成的成功率，从几乎0％提高到40％。全细胞膜片钳记录显示出延迟的向外整流电流是所有分化阶段的软骨细胞中的主要宏观电流。该电流对氯化四乙铵敏感，并且在接近K +平衡电位的膜电位下极性反转。处于静止状态的软骨细胞表达的K +电流比增生和肥大的软骨细胞小得多。当针对细胞膜面积将电流幅度标准化时，增殖细胞表达明显更高的向外电流密度。 （C）2001 Elsevier Science Inc.保留所有权利。 [参考：18]

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