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首页> 外文期刊>Life sciences >Evidence for expression of estrogen receptor cofactor messenger ribonucleic acid in the ovary and uterus of domesticated animals (sheep, cow and pig).
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Evidence for expression of estrogen receptor cofactor messenger ribonucleic acid in the ovary and uterus of domesticated animals (sheep, cow and pig).

机译：在家养动物（绵羊，牛和猪）的卵巢和子宫中表达雌激素受体辅因子信使核糖核酸的证据。

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Expression levels of estrogen receptor cofactors (coactivators or corepressors) in specific tissue compartments and cells are thought to influence the expression of estrogen responsive genes and thereby influence overall hormonal responsiveness of target tissues. To date, the presence of cofactors has been reported in tissues from humans, rats and mice. We analyzed the presence and distribution of messenger ribonucleic acids (mRNAs) encoding several transcriptional cofactors in the ovary and uterus of three domestic animal species, the sheep, cow and pig. Northern analysis for cofactors SRC-1, GRIP1, RAC3, p300, RIP140, and SPA showed expression in ovaries from all three species. In addition, lower expression of SRC-1, GRIP1, RAC3, p300, and RIP140 mRNAs was observed during the luteal phase (day 10-12 of the estrous cycle) than at estrus (day 0); however, SPA transcript levels remained unchanged. We then examined expression of mRNAs for changing (SRC-1, RIP140) and constitutively expressed (SPA) cofactors in ovine ovaries. SRC-1 and RIP140 transcripts in corpus luteum were lower compared to the surrounding ovarian tissue. SPA mRNA expression, however, was similar in corpus luteum and surrounding tissues. To determine which ovarian cell types express SRC-1, RIP140, and SPA, in situ hybridization was performed on sheep ovaries. Silver grains corresponding to these cofactors were seen in ovarian granulosa, theca and stromal cells, but appeared to be most abundant in the granulosa cells. Expression of SRC-1 and RIP140 in corpus luteum, however, was reduced compared to expression in follicular cells. Finally, we examined cofactor expression in ovine, bovine, and porcine uterus. Northern blot analysis for SRC-1, GRIP1, RAC3, p300, and RIP140 mRNAs showed higher expression in extracts of the endometrium compared to whole uterus. We provide the first evidence for the presence of estrogen receptor cofactor mRNAs in the ovary and uterus of three domestic animal species. We suggest that coactivators are conserved among species and associated with hormonal responsiveness of reproductive tract tissues in sheep, cow and pig.
机译：据认为，特定组织区室和细胞中雌激素受体辅因子（共激活因子或共加压因子）的表达水平会影响雌激素反应性基因的表达，从而影响靶组织的整体激素反应性。迄今为止，已有人，大鼠和小鼠的组织中存在辅因子的报道。我们分析了三种家畜，绵羊，牛和猪的卵巢和子宫中编码几种转录辅因子的信使核糖核酸（mRNA）的存在和分布。对辅因子SRC-1，GRIP1，RAC3，p300，RIP140和SPA的Northern分析表明，这三个物种在卵巢中都有表达。此外，在黄体期（发情周期的第10-12天）观察到的SRC-1，GRIP1，RAC3，p300和RIP140 mRNA的表达低于发情期（第0天）。但是，SPA成绩单水平保持不变。然后，我们检查了绵羊卵巢中mRNA的表达变化（SRC-1，RIP140）和组成性表达（SPA）辅助因子。黄体中的SRC-1和RIP140转录本低于周围的卵巢组织。然而，SPA mRNA在黄体和周围组织中的表达相似。为了确定哪种卵巢细胞类型表达SRC-1，RIP140和SPA，对绵羊卵巢进行了原位杂交。在卵巢颗粒，卵泡膜和基质细胞中可见到与这些辅因子相对应的银粒，但似乎在颗粒细胞中含量最高。然而，与卵泡细胞中的表达相比，SRC-1和RIP140在黄体中的表达降低了。最后，我们检查了在绵羊，牛和猪子宫中的辅因子表达。与整个子宫相比，SRC-1，GRIP1，RAC3，p300和RIP140 mRNA的Northern印迹分析显示子宫内膜提取物中的表达更高。我们提供了三种家畜的卵巢和子宫中雌激素受体辅因子mRNA的存在的第一个证据。我们建议，共激活因子在物种之间是保守的，并且与绵羊，牛和猪的生殖道组织的激素反应性有关。

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