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首页> 外文期刊>Life sciences >Inhibitor effect of omeprazole in isolated human myometrial smooth muscle
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Inhibitor effect of omeprazole in isolated human myometrial smooth muscle

机译：奥美拉唑对离体人肌平滑肌的抑制作用

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The aim of the present study was to investigate the effect of omeprazole, an H+-K+-ATPase inhibitor, in myometrial smooth muscle strips from women undergoing elective caesarean section at term. Isolated myometrial strips taken with informed consent were obtained from eight pregnant women undergoing elective caesarean section at term (not in labour) and mounted in organ baths for recording of isometric tension. We recorded the effect of increasing concentrations of omeprazole on spontaneous and Ca2+-induced contractions of myometrial smooth muscle and on contractions of myometrial smooth muscle pretreated with indomethacin (3x10(-6) M) and L-NAME (3 x 10(-5) M). Omeprazole (10(-4)-10(-3) M) decreased the amplitude and frequency of spontaneous contractions in a time- and concentration-dependent manner in all myometrial smooth muscle isolated from pregnant women. The decrease in amplitude of contractions in myometrial smooth muscle reached statistical significance beginning from the concentration of 3 x 10(-4) M, Addition of indomethacin (3 x 10(-6) M) and L-NAME (3x10(-5) M) in to the organ baths 30 min before did not change relaxation responses to omeprazole. When 8 mM Ca2+-precontracted in Ca2+-free medium myometrial smooth muscle were exposed to increasing concentrations of omeprazole (10(-5)-10(-3) M), omeprazole produced relaxation responses in a time- and concentration-dependent manner, reaching statistical significance at 10(-4) M. These results show: (1) omeprazole time- and concentration-dependently decreased spontaneous contractile activity in myometrial smooth muscle isolated from pregnant women, (2) omeprazole-induced relaxations was not influenced by indomethacin and NG-nitro-L-arginine methyl ester (L-NAME), suggesting that it is not mediated by cyclooxygenase products and nitric oxide, and (3) omeprazole brought about time- and concentration-dependently relaxation of myometrial smooth muscle precontracted by 8 mM Ca2+ in Ca2+-free medium. This effect of omeprazole may be due to blockade of the calcium channels. (C) 2001 Elsevier Science Inc. All rights reserved. [References: 23]
机译：本研究的目的是研究足月进行选择性剖宫产的妇女的子宫肌层平滑肌条中的一种H + -K + -ATPase抑制剂奥美拉唑的作用。从八名足月（不分娩）行择期剖腹产的孕妇中，经知情同意，取出孤立的子宫肌条，并安装在器官浴中以记录等距张力。我们记录了奥美拉唑浓度增加对自发和Ca2 +诱导的肌层平滑肌收缩以及吲哚美辛（3x10（-6）M）和L-NAME（3 x 10（-5）预处理）对肌层平滑肌收缩的影响M）。奥美拉唑（10（-4）-10（-3）M）以时间和浓度依赖性方式降低了从孕妇分离的所有肌层平滑肌的自发收缩幅度和频率。从3 x 10（-4）M的浓度，消炎痛（3 x 10（-6）M的添加）和L-NAME（3x10（-5）的添加开始，肌层平滑肌收缩幅度的降低达到统计学意义。 M）在30分钟前进入器官浴没有改变对奥美拉唑的松弛反应。当在不含Ca2 +的中等肌层平滑肌中预收缩的8 mM Ca2 +暴露于浓度不断增加的奥美拉唑（10（-5）-10（-3）M）时，奥美拉唑会产生时间和浓度依赖性的松弛反应，在10（-4）M时达到统计学显着性。这些结果表明：（1）奥美拉唑在时间和浓度上均依赖于从孕妇分离的子宫肌层平滑肌中的自发收缩活性，（2）奥美拉唑引起的舒张不受吲哚美辛的影响和NG-硝基-L-精氨酸甲酯（L-NAME），表明它不是由环氧合酶产物和一氧化氮介导的;（3）奥美拉唑引起时间和浓度依赖性的肌层平滑肌舒张时间是8无Ca2 +培养基中的mM Ca2 +。奥美拉唑的这种作用可能是由于钙通道的阻塞。 （C）2001 Elsevier Science Inc.保留所有权利。 [参考：23]

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