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首页> 外文期刊>Life sciences >An aqueous extract of Rubus chingii fruits protects primary rat hepatocytes against tert-butyl hydroperoxide induced oxidative stress.
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An aqueous extract of Rubus chingii fruits protects primary rat hepatocytes against tert-butyl hydroperoxide induced oxidative stress.

机译：悬钩子果实的水提取物可保护大鼠原代肝细胞免受叔丁基过氧化氢诱导的氧化应激的影响。

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摘要

Different in vitro free radical generating systems were used to assess the antioxidative activity of aqueous extracts of the five herbal components of Wu-zi-yan-zong-wan, a traditional Chinese medicinal formula with a long history of use for tonic effects. Fructus Rubi [Rubus chingii (Rosaceae) fruits] was found to be the most potent. It was further investigated using the primary rat hepatocyte system. tert-Butyl hydroperoxide (t-BHP) was used to induce oxidative stress. Being a short chain analog of lipid hydroperoxide, t-BHP is metabolized into free radical intermediates by the cytochrome P450 system in hepatocytes, which in turn, initiate lipid peroxidation, glutathione depletion and cell damage. Pre-treatment of hepatocytes with Fructus Rubi extract (50 microg/ml to 200 microg/ml) for 24 h significantly reversed t-BHP-induced cell viability loss, lactate dehydrogenase leakage and the associated glutathione depletion and lipid peroxidation. The amount of reactive oxygen species formed was also decreased as visualized by the fluorescence probe 2',7'-dichlorofluorescin diacetate. These results suggested that Fructus Rubi was useful in protecting against t-BHP-induced oxidative damage and may also be capable of attenuating cytotoxicity of other oxidants.
机译：使用了不同的体外自由基生成系统来评估五子岩宗万的五种草药成分的水提取物的抗氧化活性，这是一种具有悠久的补品功效的传统中药配方。 Rub果（Rubus chingii（蔷薇科）果实）被发现是最有效的。使用原代大鼠肝细胞系统进行了进一步研究。叔丁基氢过氧化物（t-BHP）用于诱导氧化应激。作为脂质氢过氧化物的短链类似物，t-BHP被肝细胞中的细胞色素P450系统代谢为自由基中间体，继而引发脂质过氧化，谷胱甘肽耗竭和细胞损伤。用茜草提取物（50微克/毫升至200微克/毫升）预处理肝细胞24小时，可显着逆转t-BHP诱导的细胞活力丧失，乳酸脱氢酶泄漏以及相关的谷胱甘肽耗竭和脂质过氧化。如荧光探针2′，7′-二氯荧光素二乙酸盐所观察到的，形成的活性氧种类的量也减少了。这些结果表明，F果可用于防止t-BHP引起的氧化损伤，并且还可以减弱其他氧化剂的细胞毒性。

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