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首页> 外文期刊>Cell and Tissue Research >Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies
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Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

机译：短期炎性和/或低氧预处理对牙周膜干细胞的影响：体内和体外研究

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In this study, we extensively screened the in vitro and in vivo effects of PDLSCs following short-term inflammatory and/or hypoxic pretreatments. We found that the 24-h hypoxic pretreatment of PDLSCs significantly enhanced cell migration and improved cell surface CXCR4 expression. In addition, hypoxia-pretreated PDLSCs exhibited improved cell colony formation and proliferation. Cells that were dually stimulated also formed more colonies compared to untreated cells but their proliferation did not increase. Importantly, the hypoxic pretreatment of PDLSCs enhanced cell differentiation as determined by elevated RUNX-2 and ALP protein expression. In this context, the inflammatory stimulus impaired cell OCN protein expression, while dual stimuli led to decreased RUNX-2 and OCN mRNA levels. Although preconditioning PDLSCs with inflammatory and/or hypoxic pretreatments resulted in no differences in the production of matrix proteins, hypoxic pretreatment led to the generation of thicker cell sheets; the inflammatory stimulus weakened the ability of cells to form sheets. All the resultant cell sheets exhibited clear bone regeneration following ectopic transplantation as well as in periodontal defect models; the amount of new bone formed by hypoxia-preconditioned cells was significantly greater than that formed by inflammatory stimulus- or dual-stimuli-treated cells or by nonpreconditioned cells. The regeneration of new cementum and periodontal ligaments was only identified in the hypoxia-stimulus and no-stimulus cell groups. Our findings suggest that PDLSCs that undergo short-term hypoxic pretreatment show improved cellular behavior in vitro and enhanced regenerative potential in vivo. The preconditioning of PDLSCs via combined treatments or an inflammatory stimulus requires further investigation.
机译：在这项研究中，我们广泛筛选了短期炎症和/或低氧预处理后PDLSC的体外和体内作用。我们发现PDLSCs的24小时缺氧预处理显着增强了细胞迁移并改善了细胞表面CXCR4表达。此外，缺氧预处理的PDLSCs表现出改善的细胞集落形成和增殖。与未经处理的细胞相比，受到双重刺激的细胞也形成了更多的集落，但它们的增殖并未增加。重要的是，通过升高的RUNX-2和ALP蛋白表达可以确定PDLSCs的低氧预处理可增强细胞分化。在这种情况下，炎性刺激损害了细胞OCN蛋白的表达，而双重刺激导致RUNX-2和OCN mRNA水平降低。尽管用炎症和/或低氧预处理对PDLSC进行预处理不会导致基质蛋白的产生差异，但低氧预处理却导致了较厚的细胞层的产生。炎性刺激减弱了细胞形成薄片的能力。在异位移植后以及牙周缺损模型中，所有得到的细胞片均显示出清晰的骨再生。缺氧预处理细胞形成的新骨量明显大于炎症刺激或双重刺激处理细胞或非预处理细胞形成的新骨量。仅在缺氧刺激和无刺激细胞组中鉴定出新的牙骨质和牙周膜的再生。我们的发现表明，经过短期低氧预处理的PDLSCs在体外表现出改善的细胞行为，在体内具有增强的再生潜力。通过联合治疗或炎症刺激对PDLSC进行预处理需要进一步研究。

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