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首页> 外文期刊>Life sciences >Effects of oral administration of (L)-arginine, (L)-NAME and allopurinol on intestinal ischemia/reperfusion injury in rats.
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Effects of oral administration of (L)-arginine, (L)-NAME and allopurinol on intestinal ischemia/reperfusion injury in rats.

机译：口服给予（L）-精氨酸，（L）-NAME和别嘌呤醇对大鼠肠缺血/再灌注损伤的影响。

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AIMS: Intestinal ischemia/reperfusion (I/R) injury is implicated in many clinical conditions, and it performs a fundamental role in their pathophysiologies. Oral administration of antioxidants and nitric oxide (NO) donors ameliorate intestinal injury. Here, the effects of l-arginine, allopurinol and N(G)-nitro-l-arginine methyl ester (l-NAME) were investigated. MAIN METHODS: One hundred twenty-eight male Wistar rats were separated into 4 groups and subjected to occlusion of the superior mesenteric artery for 60 min. The Control group did not receive any substance before the surgical operation. However, the 3 other groups received the following: l-arginine (800 mg/kg body weight; l-Arg group), l-NAME (50mg/kg; l-NAME group) or allopurinol (100mg/kg; Allo group). Each substance was given by mouth in 3 equal doses 24, 12 and 1h before the surgical operation. Each group was then divided into 4 subgroups, which underwent different durations of reperfusion (0, 1, 8 or 24h). At the end of each time point, blood and tissue samples were collected, and histological examinations were performed. Serum nitrite and catalase, intestinal tissue myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT) levels were determined. KEY FINDINGS: At each reperfusion time point, the Allo group exhibited the mildest histological lesions in contrast to the l-NAME group, which showed the most severe lesions. MPO was decreased significantly in the Allo and l-Arg groups during reperfusion, and allopurinol administration caused earlier and stronger effect. iNOS and NT levels were higher in the l-Arg group and lower in the Allo group. Serum nitrite and catalase were increased in the l-NAME group after 24h. SIGNIFICANCE: Oral administration of allopurinol exerted a strong and protective effect on the intestinal tissue that was subjected to I/R earlier than l-arginine. This finding was also supported with the MPO, iNOS and NT data.
机译：目的：肠缺血/再灌注（I / R）损伤与许多临床疾病有关，并且在其病理生理学中起着基本作用。口服抗氧化剂和一氧化氮（NO）供体可减轻肠道损伤。在这里，研究了l-精氨酸，别嘌呤醇和N（G）-硝基-1-精氨酸甲酯（l-NAME）的作用。主要方法：将128只雄性Wistar大鼠分成4组，并进行肠系膜上动脉闭塞60分钟。对照组在手术前未接受任何药物。但是，其他3组接受以下治疗：l-精氨酸（800 mg / kg体重; l-Arg组），l-NAME（50mg / kg; l-NAME组）或别嘌呤醇（100mg / kg; Allo组） 。在外科手术之前的24、12和1h内，以3种相等的剂量口服每种物质。然后将每组分为4个亚组，分别经历不同的再灌注持续时间（0、1、8或24h）。在每个时间点结束时，采集血液和组织样本，并进行组织学检查。测定了血清亚硝酸盐和过氧化氢酶，肠道组织髓过氧化物酶（MPO），诱导型一氧化氮合酶（iNOS）和硝基酪氨酸（NT）的水平。主要发现：在每个再灌注时间点，与l-NAME组相比，Allo组表现出最轻微的组织学病变，而l-NAME组则表现出最严重的病变。在再灌注过程中，Allo和l-Arg组的MPO显着降低，而别嘌呤醇的给药引起更早更强的作用。 l-Arg组的iNOS和NT水平较高，而Allo组的较低。 24小时后，l-NAME组的血清亚硝酸盐和过氧化氢酶升高。重要性：口服别嘌呤醇对比I-精氨酸更早进行I / R的肠组织具有强大的保护作用。 MPO，iNOS和NT数据也支持此发现。

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