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C-fos expression pattern in the hypothalamus and the preoptic area of the rat during proestrus.

机译：在发情期间，大鼠下丘脑和视前区的C-fos表达模式。

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摘要

c-fos gene expression in the hypothalamus, the preoptic area, the hippocampus and the frontal cerebral cortex of the rat was determined every two hours from 09:00 h to 19:00 h on the day of proestrus by using reverse transcription coupled to polymerase chain reaction. Rats under a 14:10 h light-dark cycle, with lights on at 06:00 h were used. We found a marked increase in c-fos gene expression in the studied regions but the hippocampus at 13:00 h, followed by a significant diminution in the subsequent hours of proestrus day. The high expression of c-fos gene at 13:00 could be associated to the increase in estradiol seric levels observed both at 11:00 h and at 13:00 h. Our results correlate with the increase in the number of FOS immunoreactive cells in some forebrain areas in proestrus afternoon related to gonadotropin releasing hormone secretion.
机译：通过与聚合酶偶联的逆转录反应，在发情日的09:00 h至19:00 h每两小时确定大鼠下丘脑，视前区，海马和额叶大脑皮层中c-fos基因的表达连锁反应。使用了在14:10 h的明暗循环下，在06:00 h点亮的大鼠。我们发现在研究区域中c-fos基因表达显着增加，但在13:00 h出现海马，随后在发情日的后续小时中显着减少。 c-fos基因在13:00时的高表达可能与在11:00和13:00时观察到的雌二醇浆液水平的升高有关。我们的结果与在前发性下午某些前脑区域FOS免疫反应性细胞数量的增加有关，这与促性腺激素释放激素的分泌有关。

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