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CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF RABBIT ALPHA-1A-ADRENOCEPTOR

机译:兔α-1A-肾上腺受体的克隆,功能表达及组织分布

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摘要

A cDNA clone, which has an open reading frame of 1398 nucleotides encoding a 466-amino-acid peptide, has been isolated from rabbit liver cDNA library. Compared with the peptide sequence, it shows high homology to ala adrenoceptors of human, bovine and rat. We expressed this clone in COS-7 and investigated the pharmacological properties, revealing similarity to those of human ala adrenoceptors. Competitive RT/PCR has detected the mRNA in variety of rabbit tissues, especially abundantly in liver, vas deferens, brain, and aorta, but not in heart. [References: 22]
机译:从兔肝cDNA文库中分离出一个cDNA克隆,该克隆具有1398个核苷酸的开放阅读框,编码466个氨基酸肽。与肽序列相比,它与人,牛和大鼠的ala肾上腺素受体具有高度同源性。我们在COS-7中表达了该克隆,并研究了其药理特性,揭示出与人类ala肾上腺素受体相似的特性。竞争性RT / PCR已检测到各种兔子组织中的mRNA,尤其是在肝脏,输精管,大脑和主动脉中,但在心脏中却没有。 [参考:22]

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