Position 120-123, a potential active site of trichosanthin.

Nie H; Cai X; He X; Xu L; Ke X; Ke Y; Tam SC

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Position 120-123, a potential active site of trichosanthin.

机译：位置120-123，天花粉蛋白的潜在活性位点。

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Trichosanthin (TCS) is a type-I ribosome-inactivating protein (RIP) with wide spectrum of biological and pharmacological activities. In the present study, a potential site on the TCS molecule (position 120-123) is identified which may be important for the biological activities of TCS. By using site-directed mutagenesis, position 120-123 of TCS was either deleted or changed from Lys-Ile-Arg-Glu (hydrophilic) to Ser-Ala-Gly-Gly (hydrophobic). Deletion of these residues rendered a TCS molecule completely deprived of ribosome inactivating activity, while hydrophobic replacement caused 4000-fold decrease in ribosome inactivating activity. The abortifacient activity of these two mutants was retained with decreased potency. This implies that position 120-123 of the native TCS molecule plays a critical role in maintaining its biological activity.

机译：天花粉蛋白（TCS）是一种I型核糖体失活蛋白（RIP），具有广泛的生物学和药理活性。在本研究中，确定了TCS分子上的潜在位点（位置120-123），这对于TCS的生物学活性可能很重要。通过使用定点诱变，TCS的位置120-123被删除或从Lys-Ile-Arg-Glu（亲水）变为Ser-Ala-Gly-Gly（疏水）。这些残基的缺失使得TCS分子完全丧失了核糖体的失活活性，而疏水性置换导致核糖体的失活活性降低了4000倍。这两个突变体的堕胎活性得以保留，但效力降低。这暗示着天然TCS分子的120-123位在维持其生物活性中起关键作用。

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《Life sciences》 |1998年第6期|共10页
作者
Nie H; Cai X; He X; Xu L; Ke X; Ke Y; Tam SC;
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正文语种 eng
中图分类生命的起源;生命科学总论;
关键词
Abortifacient Agents; Nonsteroidal; Trichosanthin; 堕胎药; 非甾类; 天花粉素;

机译：Abortifacient Agents;Nonsteroidal;Trichosanthin;堕胎药;非甾类;天花粉素;

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1. Position 120-123, a potential active site of trichosanthin. [J] . Nie H, Cai X, He X, Life sciences . 1998,第6期

机译：位置120-123，天花粉蛋白的潜在活性位点。
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3. The P4 metal binding site in RNase P RNA affects active site metal affinity through substrate positioning [J] . Christian EL, Smith KMJ, Perera N, RNA . 2006,第8期

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4. Surface Morphology and Chemically Active Sites on Ru Based Ultracapacitors: Montecarlo Simulation with Embedded Atom Potentials [C] . Lesser Blum, Marc D Legault, Fredy R Zypman 1999 International Conference on Modeling and Simulation of Microsystems Apr 19-21, 1999, San Juan, Puerto Rico, USA . 1999

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5. Effects of N-2-acetylaminofluorene (AAF) and 2-aminofluorene (AF) adducts positioned in the active site of replicative polymerases on nucleotide and polymerase binding. [D] . Ullah, Asad. 2008

机译：位于复制性聚合酶活性位点的N-2-乙酰氨基芴（AAF）和2-氨基芴（AF）加合物对核苷酸和聚合酶结合的影响。
6. The P4 metal binding site in RNase P RNA affects active site metal affinity through substrate positioning [O] . Eric L. Christian, Kari M.J. Smith, Nicholas Perera, 2006

机译：RNase P RNA中的P4金属结合位点通过底物定位影响活性位点金属亲和力
7. Protein engineering of active site residues of trichosanthin. [O] . 1993

机译：protein engineering of active site residues of trichosanthin.

Position 120-123, a potential active site of trichosanthin.

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