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首页> 外文期刊>Life sciences >Alpha-adrenoceptor subtypes of dog saphenous vein: another unusual property.
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Alpha-adrenoceptor subtypes of dog saphenous vein: another unusual property.

机译：狗大隐静脉的α-肾上腺素受体亚型：另一个不寻常的特性。

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摘要

The functional relationship between vascular smooth muscle alpha1- and alpha2-adrenoceptor (AR) subtypes was investigated by simultaneous measurement of contractile and fluorescence ratio in fura-2 loaded rings of dog saphenous vein (DSV). Prazosin, as well as rauwolscine, at 0.1 microM, substantially antagonized contractions and associated cytosolic [Ca2+] rises induced by UK 14304, while rauwolscine, as well as prazosin, antagonized similar effects of phenylephrine (PE). These antagonisms were characterized by a parallel rightward shift of the concentration-response curves. In the absence of extracellular Ca2+, PE as well as UK 14304 caused simultaneous transient elevation of contractile force and cytosolic [Ca2+], although the UK 14304 responses were smaller than PE responses. We propose that DSV smooth muscle cells possess interacting alpha1- and alpha2-ARs which have overlapping functional domain sensitive to the agonists and antagonists of either alpha-AR subtype. Both alpha-AR subtypes appear to utilize similar signaling mechanisms via Ca2+ release from the same intracellular stores and Ca2+ entry across the plasma membrane.
机译：通过同时测量犬大隐静脉（DSV）的fura-2加载环中的收缩率和荧光比，研究了血管平滑肌α1-和α2-肾上腺素能受体（AR）亚型之间的功能关系。 UK 14304诱导的Prazosin和劳乌斯汀浓度为0.1 microM，可显着拮抗收缩作用，并伴随相关的胞质[Ca2 +]升高，而劳乌斯辛以及prazosin则拮抗去氧肾上腺素（PE）的类似作用。这些拮抗作用的特征是浓度-反应曲线平行向右移动。在缺乏细胞外Ca2 +的情况下，PE和UK 14304引起收缩力和胞质[Ca2 +]的同时瞬时升高，尽管UK 14304的反应小于PE的反应。我们建议DSV平滑肌细胞具有相互作用的α1-和α2-AR，它们具有对α-AR亚型的激动剂和拮抗剂敏感的重叠功能域。两种α-AR亚型似乎都通过相同的细胞内存储中的Ca2 +释放和Ca2 +跨质膜进入而利用了类似的信号传导机制。

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