• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Adrenocorticotropic hormone controls Concanavalin A activation of rat lymphocytes by modulating IL-2 production
【24h】

Adrenocorticotropic hormone controls Concanavalin A activation of rat lymphocytes by modulating IL-2 production

机译：促肾上腺皮质激素通过调节IL-2的产生来控制伴刀豆球蛋白A对大鼠淋巴细胞的激活

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The initiation of DNA synthesis and secretion of Interleukin 2 (IL-2) was measured in isolated rat splenic lymphocytes following activation with Concanavalin A (ConA). The extent of H-3-thymidine incorporation into activated cells was tested when cultured with various concentrations of Adrenocorticotropic hormone (ACTH), A paradoxical dose-response curve resulted when ACTH caused a biphasic response of augmenting and inhibiting 3H-thymidine uptake in lymphocytes depending on the hormone concentration. Low levels of ACTH (0,001-1-nM) augmented 3H-thymidine uptake and high levels (10-1000 nM) reversed the effect.. The optimal ACTH concentration was 10 pM ACTH in the presence of 5 mu g/ml ConA and there was no ACTH effect on quiescent cells (no ConA), Conditioned media from splenic lymphocytes treated with various concentrations of ConA or ACTH was tested for increased uptake of H-3-thymidine by the IL-2 growth dependent Cytotoxic T Lymphocyte Leukemia (CTLL-2) cells. ConA conditioned medium could sustain the CTLL-2 cells indicating the presence of IL-2, Conditioned medium from splenic lymphocytes treated with both ConA and 100 pM ACTH fur ther increased CTLL-2 cell proliferation indicating an additional increase of IL-2 secretion. The identity of IL-2 was confirmed by using an anti-rat IL-2 antibody to neutralize the growth potential of the conditioned medium. ACTH alone had no effect on the CTLL-2 cell proliferation indicating the effect is due solely to induced IL-2 found in the conditioned medium. IL-2 levels in the conditioned media were quantitated by ELISA assay; splenic lymphocytes produced 4.2 ng/ml to ConA only, 19.2 ng/ml in ConA plus 10 nM ACTH, and no detectable IL-2 at ConA plus 10 uM ACTH. These results demonstrated that ACTH modulates IL-2 secretion from activated lymphocytes, which is both biphasic and concentration dependent. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 23]
机译：在用伴刀豆球蛋白A（ConA）激活后，在分离的大鼠脾淋巴细胞中测量了DNA合成的启动和白介素2（IL-2）的分泌。当用各种浓度的促肾上腺皮质激素（ACTH）培养时，测试了H-3-胸苷掺入活化细胞的程度。当ACTH引起淋巴细胞增加和抑制3H-胸苷摄取的两相反应时，产生了自相矛盾的剂量反应曲线，具体取决于关于激素的浓度。低水平的ACTH（0.001-1nM）会增加3H-胸腺嘧啶核苷的摄取，而高水平的（10-1000 nM）会逆转这种影响。在5μg / ml ConA存在的情况下，最佳ACTH浓度为10 pM ACTH。对静止细胞没有ACTH效应（没有ConA），用IL-2生长依赖性细胞毒性T淋巴细胞白血病（CTLL-）测试了用各种浓度的ConA或ACTH处理的脾淋巴细胞的条件培养基对H-3-胸苷的摄取增加2）细胞。 ConA条件培养基可以维持CTLL-2细胞，表明存在IL-2，ConA和100​​ pM ACTH处理的脾淋巴细胞条件培养基进一步增加CTLL-2细胞增殖，表明IL-2分泌进一步增加。通过使用抗大鼠IL-2抗体中和条件培养基的生长潜力，可以确认IL-2的身份。单独的ACTH对CTLL-2细胞增殖没有影响，表明该作用仅是由于条件培养基中存在诱导的IL-2引起的。通过ELISA测定定量条件培养基中的IL-2水平。脾淋巴细胞仅对ConA产生4.2 ng / ml，在ConA加10 nM ACTH中产生19.2 ng / ml，在ConA加10 uM ACTH时未检测到IL-2。这些结果表明ACTH调节了活化淋巴细胞的IL-2分泌，这是双相的和浓度依赖性的。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：23]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号