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首页> 外文期刊>Life sciences >Expressions of putative neurotransmitters and neuronal growth related genes in Merkel cell-neurite complexes of the rats.
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Expressions of putative neurotransmitters and neuronal growth related genes in Merkel cell-neurite complexes of the rats.

机译：推测的神经递质和神经元生长相关基因在大鼠默克尔细胞神经突复合物中的表达。

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Reverse transcription-polymerase chain reaction using 32P-labeled dCTP with specific primers for putative neurotransmitters related and neuronal growth-related genes (GAP-43, NGF and BDNF) were used to search for evidence of such substances in the Merkel cell. Merkel cell samples were made from sinus hairs in the facial skin of rats. The relative amount of mRNA in a tissue sample concentrated in Merkel cells was compared semiquantitatively to that from nearby tissue without Merkel cells. mRNAs for VIP, tyrosine hydroxylase, substance P were found in higher concentration in Merkel cells than in control tissues. mRNA for genes encoding pro-enkephalin, GAP-43, CGRP, NGF and BDNF were detected in the Merkel cell samples at concentration statistically equivalent to those found in the control tissues. It was concluded that the relative concentration of mRNAs for VIP, tyrosine hydroxylase and substance P is consistent with the possibility that Merkel cell acts as a possible transduction element in mechanical excitation of sense organs in which Merkel cells are present.
机译：逆转录聚合酶链反应使用32P标记的dCTP与特定的引物对推定的神经递质相关和神经元生长相关的基因（GAP-43，NGF和BDNF）进行了反演，以寻找默克尔细胞中此类物质的证据。默克尔细胞样品是由大鼠面部皮肤的窦毛制成的。将半数浓缩的默克细胞中的组织样品中mRNA的相对量与附近没有默克尔细胞的组织中的mRNA相对比。在默克尔细胞中发现VIP，酪氨酸羟化酶和P物质的mRNA浓度高于对照组织。在默克尔细胞样品中检测到编码前脑啡肽，GAP-43，CGRP，NGF和BDNF的基因的mRNA，其浓度在统计学上与对照组织相同。结论是VIP，酪氨酸羟化酶和P物质的mRNA相对浓度与默克尔细胞在存在默克尔细胞的感觉器官的机械激发中充当可能的转导元件的可能性一致。

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