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Sativin: a novel antifungal miraculin-like protein isolated from legumes of the sugar snap Pisum sativum var. macrocarpon.

机译:Sativin:一种新的抗真菌奇迹蛋白样蛋白,从糖食豌豆(Pisum sativum var)的豆类中分离出来。大果。

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摘要

An antifungal protein designated sativin was isolated from the legumes of the sugar snap (also known as honey pea) Pisum sativum var. macrocarpon. The procedure entailed extraction, affinity chromatography on Affi-gel blue gel and ion exchange chromatography on CM-Sepharose. The protein exhibited a molecular weight of 38 kDa in SDS-polyacrylamide gel electrophoresis. It possessed an N-terminal amino acid sequence which showed similarity to those of miraculin (a sweet protein) and pisavin (a ribosome-inactivating protein from Pisum sativum var arvense Poir manifesting similarity to miraculin). Unlike pisavin, however, sativin demonstrated negligible ribonuclease activity and inhibited translation in a rabbit reticulocyte lysate system with a very low potency (IC50= 14 microM). Sativin exerted antifungal activity against Fusarium oxysporum, Coprinus comatus and Pleurotus ostreatus but not against Rhizoctonia solani.
机译:从糖荚(豆荚)Pisum sativum var的豆科植物中分离出一种名为sativin的抗真菌蛋白。大果。该过程包括萃取,Affi-gel蓝色凝胶上的亲和色谱和CM-Sepharose上的离子交换色谱。在SDS-聚丙烯酰胺凝胶电泳中,该蛋白质的分子量为38 kDa。它具有一个N端氨基酸序列,该序列与奇迹蛋白(一种甜蛋白)和比萨凡醇(一种来自Pisum sativum var arvense Poir的核糖体失活蛋白,与奇迹蛋白相似)具有相似性。然而,与比萨沙文不同,萨蒂文在兔网织红细胞裂解液系统中的核糖核酸酶活性可忽略不计,并且翻译效率非常低(IC50 = 14 microM)。 Sativin对尖孢镰刀菌,鸡腿菇和平菇都具有抗真菌活性,但对茄红霉菌却没有。

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