• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Quantitative and qualitative gender-related differences in jejunal glutathione S-transferase in the rat - Effect of testosterone administration
【24h】

Quantitative and qualitative gender-related differences in jejunal glutathione S-transferase in the rat - Effect of testosterone administration

机译：大鼠空肠谷胱甘肽S-转移酶性别相关的数量和质量差异-睾丸激素的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gender-related differences and the regulation by testosterone of glutathione S-transferase were studied in rat jejunum. We analyzed enzyme activity and the relative content of GST subunits. Four experimental groups of adult rats were studied: normal males, castrated males, castrated males injected with testosterone and normal females. Glutathione S-transferase activity was assayed using 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates. Differences in subunit composition among groups were evaluated by western blot analysis. The results demonstrated that 1-chloro-2,4-dinitrobenzene conjugation rate is higher in normal males than in normal females and castrated males. Testosterone administration to castrated males raised the activity up to the level observed in normal males. No significant difference in glutathione S-transferase activity towards 1,2-dichloro-4-nitrobenzene was observed among groups. Western blot analysis revealed that males and females differ in all subunits tested that is, rGSTA2, rGSTM1, rGSTM2 and rGSTP1, and that testosterone regulates the content of rGSTM1, rGSTM2 and rGSTP1. In conclusion, jejunal GST shows a gender-dependent regulation affecting both enzyme activity and subunit composition, and testosterone appears to be one of the factors involved. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 30]
机译：研究了大鼠空肠中性别相关的差异和谷胱甘肽S-转移酶的睾丸激素调节。我们分析了酶活性和GST亚基的相对含量。研究了四个成年大鼠实验组：正常雄性，去势雄性，注射睾丸激素的去势雄性和正常雌性。以1-氯-2,4-二硝基苯和1,2-二氯-4-硝基苯为底物测定谷胱甘肽S-转移酶活性。通过蛋白质印迹分析评估各组之间亚基组成的差异。结果表明，正常男性的1-氯-2,4-二硝基苯结合率高于正常女性和and割的男性。向cast割的雄性动物服用睾丸激素可使该活性达到正常雄性动物所观察到的水平。在各组之间，谷胱甘肽S-转移酶对1,2-二氯-4-硝基苯的活性没有显着差异。蛋白质印迹分析表明，雄性和雌性在所有测试的亚基rGSTA2，rGSTM1，rGSTM2和rGSTP1中均不同，而睾丸激素调节rGSTM1，rGSTM2和rGSTP1的含量。总之，空肠GST显示出影响酶活性和亚基组成的性别依赖性调节，而睾丸激素似乎是其中的因素之一。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：30]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号