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首页> 外文期刊>Life sciences >Inhibition of the oligosaccharides extracted from Morinda officinalis, a Chinese traditional herbal medicine, on the corticosterone induced apoptosis in PC12 cells.
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Inhibition of the oligosaccharides extracted from Morinda officinalis, a Chinese traditional herbal medicine, on the corticosterone induced apoptosis in PC12 cells.

机译：从巴戟天提取的低聚糖对皮质酮诱导的PC12细胞凋亡具有抑制作用。

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摘要

The mechanisms of the antidepressant action of the oligosaccharides (P(6)) extracted from Morinda Officinalis were studied. By flow cytometry analysis, treatment of PC12 cells with corticosterone (Cort) induced apoptosis in a concentration and time dependent manner. The highest percentage of apoptotic cells accumulated to 27.85 +/- 9.2% following pretreatment with Cort 10 microM for 5 d. In agarose gel electrophoresis of DNA, the sample obtained from PC12 cells pretreated with Cort 10 microM for 5 d showed a typical ladder pattern suggesting that Cort increased the DNA fragmentation significantly. Furthermore, the ultrastructure of Cort-treated cells displayed typical apoptosis-like morphological changes including fragmented chromatin accumulation to the inside of nucleolus membrane with a shape like crescent moon or ring, nuclear fragmentation or apoptotic body. In the presence of P(6), or tricyclic antidepressant desipramine (DIM), the apoptosis induced by Cort in the three measurements above was significantly inhibited. These results indicate that DIM or P(6) antagonize the apoptosis induced by Cort in PC12 cells, which may be one of the cellular mechanisms of their antidepressant effects.
机译：研究了从巴戟天提取的寡糖（P（6））的抗抑郁作用机理。通过流式细胞仪分析，用皮质酮（Cort）处理PC12细胞以浓度和时间依赖性方式诱导凋亡。在用Cort 10 microM预处理5 d后，凋亡细胞的最高百分比累积至27.85 +/- 9.2％。在DNA的琼脂糖凝胶电泳中，从用Cort 10 microM预处理5 d的PC12细胞获得的样品显示出典型的梯形图，表明Cort显着增加了DNA片段化。此外，用Cort处理的细胞的超微结构表现出典型的凋亡样形态变化，包括染色质碎片堆积在核仁膜内部，呈月牙形或月牙形，核碎片或凋亡小体。在存在P（6）或三环抗抑郁药地昔帕明（DIM）的情况下，在上述三个测量中由Cort诱导的细胞凋亡被显着抑制。这些结果表明，DIM或P（6）拮抗Cort诱导PC12细胞凋亡，这可能是其抗抑郁作用的细胞机制之一。

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