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首页> 外文期刊>Life sciences >ATP and ADP hydrolysis in brain membranes of zebrafish (Danio rerio).
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ATP and ADP hydrolysis in brain membranes of zebrafish (Danio rerio).

机译：斑马鱼（Danio rerio）脑膜中的ATP和ADP水解。

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摘要

Nucleotides, e.g. ATP and ADP, are important signaling molecules, which elicit several biological responses. The degradation of nucleotides is catalyzed by a family of enzymes called NTPDases (nucleoside triphosphate diphosphohydrolases). The present study reports the enzymatic properties of a NTPDase (CD39, apyrase, ATP diphosphohydrolase) in brain membranes of zebrafish (Danio rerio). This enzyme was cation-dependent, with a maximal rate for ATP and ADP hydrolysis in a pH range of 7.5-8.0 in the presence of Ca(2+) (5 mM). The enzyme displayed a maximal activity for ATP and ADP hydrolysis at 37 degrees C. It was able to hydrolyze purine and pyrimidine nucleosides 5'-di and triphosphates, being insensitive to classical ATPase inhibitors, such as ouabain (1 mM), N-ethylmaleimide (0.1 mM), orthovanadate (0.1 mM) and sodium azide (0.1 mM). A significant inhibition of ATP and ADP hydrolysis (68% and 34%, respectively) was observed in the presence of 20 mM sodium azide, used as a possible inhibitor of ATP diphosphohydrolase. Levamisole (1 mM) and tetramisole (1 mM), specific inhibitors of alkaline phosphatase and P1, P(5)-di (adenosine 5'-) pentaphosphate, an inhibitor of adenylate kinase did not alter the enzyme activity. The presence of a NTPDase in brain membranes of zebrafish may be important for the modulation of nucleotide and nucleoside levels, controlling their actions on specific purinoceptors in central nervous system of this specie.
机译：核苷酸，例如ATP和ADP是重要的信号分子，可引起多种生物学反应。核苷酸的降解被称为NTPDases（三磷酸核苷二磷酸水解酶）的一族酶催化。本研究报告了斑马鱼（Danio rerio）脑膜中NTPDase（CD39，腺苷三磷酸酶，ATP二磷酸水解酶）的酶学性质。此酶是阳离子依赖性的，在Ca（2+）（5 mM）存在的情况下，在7.5-8.0的pH范围内，ATP和ADP水解的最大速率。该酶在37摄氏度下显示最大的ATP和ADP水解活性。它能够水解嘌呤和嘧啶核苷5'-二和三磷酸，对经典ATPase抑制剂（如哇巴因（1 mM），N-乙基马来酰亚胺）不敏感（0.1 mM），原钒酸盐（0.1 mM）和叠氮化钠（0.1 mM）。在存在20 mM叠氮化钠的情况下，可以显着抑制ATP和ADP水解（分别为68％和34％），叠氮化钠可用作ATP二磷酸水解酶的抑制剂。 Levamisole（1 mM）和tetramisole（1 mM），碱性磷酸酶和P1，P（5）-di（腺苷5'-）五磷酸的特异性抑制剂，腺苷酸激酶的抑制剂不会改变酶的活性。斑马鱼脑膜中NTPDase的存在对于调节核苷酸和核苷水平，控制它们对该物种中枢神经系统中特定嘌呤受体的作用可能很重要。

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