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首页> 外文期刊>Life sciences >A study on the localization and distribution of GnRH and its receptor in rat submaxillary glands by immunohistochemical, in situ hybridization and RT-PCR.
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A study on the localization and distribution of GnRH and its receptor in rat submaxillary glands by immunohistochemical, in situ hybridization and RT-PCR.

机译：通过免疫组织化学，原位杂交和RT-PCR研究GnRH及其受体在大鼠颌下腺中的定位和分布。

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摘要

We investigated the rat submaxillary gland for the presence of GnRH and GnRH receptors, the localization and colocalization of GnRH, GnRH receptor and their mRNA, and studied the sequence of GnRH receptor complementary DNA (cDNA) by immunohistochemistry, in situ hybridization and RT-PCR. The results showed that GnRH and GnRH receptor immunoreactive materials were colocalized in the epithelial cells of the serous acinus and glandular duct. The GnRH and GnRH receptor mRNA hybridization signals were detected in the above cells. The sequence obtained from the RT-PCR product was identical to the published cDNA sequence of GnRH receptor in the rat pituitary. The results suggested that the rat submaxillary gland was capable of synthesizing GnRH and GnRH receptors. GnRH may be involved in the functional regulation of the submaxillary gland through autocrine or paracrine activity.
机译：我们研究了大鼠上颌下腺中GnRH和GnRH受体的存在，GnRH，GnRH受体及其mRNA的定位和共定位，并通过免疫组织化学，原位杂交和RT-PCR研究了GnRH受体互补DNA（cDNA）的序列。 。结果表明，GnRH和GnRH受体免疫反应材料共定位于浆液性腺和腺管的上皮细胞中。在上述细胞中检测到GnRH和GnRH受体mRNA杂交信号。从RT-PCR产物获得的序列与大鼠垂体中GnRH受体的已公开cDNA序列相同。结果表明，大鼠上颌下腺能够合成GnRH和GnRH受体。 GnRH可能通过自分泌或旁分泌活性参与上颌下腺的功能调节。

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