...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Misexpression of mouse porcupine isoforms modulates the differentiation of P19 embryonic carcinoma cells.
【24h】

Misexpression of mouse porcupine isoforms modulates the differentiation of P19 embryonic carcinoma cells.

机译：小鼠豪猪同工型的错误表达调节P19胚胎癌细胞的分化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Drosophila porcupine (porc) encodes an ER membrane protein that is required for the processing of the Drosophila Wnt family. Homologs of porc have been identified in various multicellular organisms and have been implicated in the biosynthesis of Wnt proteins. In contrast to Drosophila, vertebrates generate four different porc mRNAs (A-D) by alternative splicing. Murine porcD (MporcD) mRNA levels transiently increase during the neuroectodermal differentiation of P19 cells, but diminish during mesodermal differentiation. P19 cells constitutively expressing mouse porcA (MporcA), but not MporcD, undergo apoptosis by the induction of neuroectodermal differentiation. Meanwhile, P19 cells constitutively expressing MporcD, but not MporcA, do not adopt mesodermal cell morphology and fail to express myf-5 when induced to mesodermal differentiation. These results therefore demonstrate that the alternative splicing of Mporc is regulated in a cell-type specific manner, and the resulting Mporc isoforms have different functions in the neuroectodermal and mesodermal differentiation of P19 cells.
机译：果蝇豪猪（porc）编码果蝇Wnt家族加工所需的ER膜蛋白。已在多种多细胞生物中鉴定出了porc的同源物，并与Wnt蛋白的生物合成有关。与果蝇相反，脊椎动物通过交替剪接产生四种不同的porc mRNA（A-D）。小鼠porcD（MporcD）mRNA水平在P19细胞的神经外胚层分化过程中瞬时增加，但在中胚层分化过程中降低。组成性表达小鼠porcA（MporcA）但不表达MporcD的P19细胞通过诱导神经外胚层分化而发生凋亡。同时，组成性表达MporcD而不是MporcA的P19细胞不采用中胚层细胞形态，并且在诱导中胚层分化时不能表达myf-5。因此，这些结果表明，Mporc的选择性剪接受到细胞类型特异性方式的调控，并且所得的Mporc亚型在P19细胞的神经外胚层和中胚层分化中具有不同的功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号