• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Enhancement of alkaline phosphatase synthesis in pulp cells co-cultured with epithelial cells derived from lower rabbit incisors.
【24h】

Enhancement of alkaline phosphatase synthesis in pulp cells co-cultured with epithelial cells derived from lower rabbit incisors.

机译：与来自下兔门牙的上皮细胞共培养的牙髓细胞中碱性磷酸酶合成的增强。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dental papilla mesenchymal cells differentiate into odontoblasts through epithelial-mesenchymal interactions. However, the mechanism by which enamel epithelial cells affect the differentiation of dental mesenchymal cells remains unknown. Alkaline phosphatase (ALPase) is a marker for odontoblast-like differentiation, because odontoblasts show much higher ALPase activity than dental undifferentiated mesenchymal cells. The continuously growing rabbit incisor is a good model for the epithelial-mesenchymal interaction during odontogenesis. In the present study, we isolated and maintained rabbit incisor-derived epithelial cells and rabbit incisor pulp-derived fibroblastic cells, and examined the effect of epithelial cells on ALPase activity in fibroblastic cells. Epithelial cells were stained with anti-cytokeratin 5 and 8 antibodies and showed the expression of tuftelin mRNA. In separate cultures of epithelial cells or fibroblastic cells, ALPase activity and mRNA levels were very low, but were upregulated inco-cultures of epithelial and fibroblastic cells. Histochemical analysis found high ALPase activity in fibroblastic cells close to epithelial cells. These findings suggest that epithelial cells play an important role in promoting ALPase expression in pulp fibroblastic cells. The co-culture system developed here will be useful for examining the role of the epithelial-mesenchymal interaction during odontoblast differentiation.
机译：牙乳头间充质细胞通过上皮-间质相互作用分化为成牙本质细胞。然而，釉质上皮细胞影响牙齿间充质细胞分化的机制仍然未知。碱性磷酸酶（ALPase）是成牙本质细胞样分化的标志，因为成牙本质细胞显示出比牙齿未分化的间充质细胞高得多的ALPase活性。不断增长的兔门牙是成牙过程中上皮-间质相互作用的良好模型。在本研究中，我们分离并维持了兔切牙来源的上皮细胞和兔切牙牙髓来源的成纤维细胞，并研究了上皮细胞对成纤维细胞中ALPase活性的影响。上皮细胞用抗细胞角蛋白5和8抗体染色并显示Tuftelin mRNA的表达。在上皮细胞或成纤维细胞的单独培养物中，ALPase活性和mRNA水平非常低，但是上皮和成纤维细胞的共培养中上调。组织化学分析发现，在接近上皮细胞的成纤维细胞中，ALPase活性较高。这些发现表明，上皮细胞在促进牙髓成纤维细胞中的ALPase表达中起重要作用。这里开发的共培养系统将有助于检查成牙本质细胞分化过程中上皮-间质相互作用的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号