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首页> 外文期刊>Cell biology international. >Actin expression in Taenia solium cysticerci (cestoda): tisular distribution and detection of isoforms.
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Actin expression in Taenia solium cysticerci (cestoda): tisular distribution and detection of isoforms.

机译：en虫（Taenia cysticerci）（cestoda）中肌动蛋白的表达：囊状分布和同工型检测。

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摘要

Identification, localization and partial biochemical characterization of actins expressed in the larval stage of the cestode parasite Taenia solium has been carried out. Frozen tissue sections of cysticerci, the larval stage of this parasite, were reacted with rhodamine-phalloidin, parasite actin was purified by polymerization in the presence of K+, mg++and ATP actin was analyzed by SDS-PAGE and two-dimensional gel electrophoresis, and immunoblotting of actin was performed in PVDF membranes and with commercial anti-actin monoclonal antibodies. Parasitic tissues showed different fibrous actin fluorescence patterns, which correlated with the expression of isoactins. Purified globular actin had a similar molecular mass to rabbit commercial actin ( approximately 44 kDa). Actin was resolved into seven isoforms, indicating a family of actin genes.
机译：est虫寄生虫Ta虫Ta虫幼虫期表达的肌动蛋白的鉴定，定位和部分生化特性已经完成。囊虫的冷冻组织切片，即该寄生虫的幼虫阶段，与若丹明-鬼笔环肽反应，在K +，mg ++存在下通过聚合纯化寄生虫肌动蛋白，并通过SDS-PAGE和二维凝胶电泳分析ATP肌动蛋白，肌动蛋白的免疫印迹在PVDF膜上进行，并使用市售的抗肌动蛋白单克隆抗体进行。寄生虫组织显示出不同的纤维肌动蛋白荧光模式，这与异肌动蛋白的表达相关。纯化的球状肌动蛋白具有与兔市售肌动蛋白相似的分子量（约44 kDa）。肌动蛋白被分解为七个同工型，表明肌动蛋白基因家族。

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