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首页> 外文期刊>Cell biology international. >Time and cell cycle dependent formation of heterogeneous tubulin arrays induced by colchicine in Triticum aestivum root meristem.
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Time and cell cycle dependent formation of heterogeneous tubulin arrays induced by colchicine in Triticum aestivum root meristem.

机译：时间和细胞周期依赖性形成的普通小麦根分生组织中秋水仙碱诱导的异质微管蛋白阵列的形成。

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摘要

We have investigated the appearance and reorganization of tubulin-containing arrays induced by colchicine in the root meristem of wheat Triticum aestivum, using immunostaining and electron microscopy. Colchicine caused depolymerization of microtubules and formation of tubulin cortical strands composed of filamentous material only in C-mitotic cells. After prolonged exposure to the drug, both interphase and C-mitotic cells acquired needle-type bundles, arranged as different crystalloids and/or macrotubules. The unmodified tyrosinated form of alpha-tubulin was detected within microtubules in control cells, but was not found within cortical strands. It was identified, however, within needle-type bundles. The modified acetylated form of alpha-tubulin, which was absent in control cells, was detected within needle-type bundles. Thus, cortical strands were transitory arrays, transformed into needle-type bundles during prolonged exposure to colchicine. Cortical strands appeared in a cell cycle-dependent manner, whereas needle-type bundles were cell cycle stable arrays. The diverse morphological organization, intracellular distribution and stability of tubulin-containing arrays may be associated with heterogeneity of alpha-tubulin isoforms. We assume that non-microtubular arrays substitute for microtubules in conditions where normal tubulin polymerization is inhibited.
机译：我们使用免疫染色和电子显微镜研究了秋水仙碱诱导的小麦小麦根分生组织中含微管蛋白的阵列的重组。秋水仙碱仅在C有丝分裂细胞中引起微管解聚并形成由丝状物质组成的微管蛋白皮质链。长时间暴露于药物后，相间和C-有丝分裂细胞均获得针状束，排列成不同的晶体和/或大管。在对照细胞的微管中检测到未修饰的酪氨酸化形式的α-微管蛋白，但在皮质链中未发现。但是，在针型束中发现了它。在针型束中检测到对照细胞中不存在的α-微管蛋白的修饰乙酰化形式。因此，皮质链是短暂的阵列，在长时间暴露于秋水仙碱期间转变成针状束。皮质链以细胞周期依赖性方式出现，而针型束是细胞周期稳定的阵列。含微管蛋白的阵列的不同形态组织，细胞内分布和稳定性可能与α-微管蛋白同工型的异质性有关。我们假设在正常微管蛋白聚合受到抑制的条件下，非微管阵列可替代微管。

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