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首页> 外文期刊>Cell biology international. >Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry.
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Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry.

机译：丝裂霉素-C对用作人类胚胎干细胞饲养者的包皮成纤维细胞的影响：免疫细胞化学MIB1评分和DNA倍性及细胞凋亡通过流式细胞术评估。

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摘要

Mitomycin C (MMC) treatment has been used to arrest cell proliferation but not much is known about the effect of MMC on human foreskin fibroblasts (HFF) used as feeders for human embryonic stem cells (hESC). We tested the ability of MMC to stop the proliferation of HFF and to induce apoptosis. MMC inhibited the proliferation of HFF at 10 microg/ml over 2.5h of MMC treatment showing a decrease in the proliferation index measured by Ki-67 and S and G2/M phases related to active HFF. A low percentage of cells showed necrotic or apoptotic features using different lengths of incubation. Over time, the majority of cells remained in a mitotically inactive state. The percentage of apoptotic cells increased from day 2 to day 10, at the same time as the necrotic ones increased. The HS181 hESC line grew in an undifferentiated state on inactive HFF throughout the study.
机译：丝裂霉素C（MMC）处理已被用于阻止细胞增殖，但对于MMC对用作人类胚胎干细胞（hESC）饲养者的人类包皮成纤维细胞（HFF）的影响知之甚少。我们测试了MMC阻止HFF增殖并诱导凋亡的能力。 MMC在2.5h的MMC处理中以10 microg / ml的速度抑制HFF的增殖，显示通过与活性HFF相关的Ki-67和S和G2 / M期测定的增殖指数降低。使用不同的孵育时间，低百分比的细胞显示出坏死或凋亡特征。随着时间的流逝，大多数细胞保持在有丝分裂失活状态。从第2天到第10天，凋亡细胞的百分比增加，而坏死细胞的百分比增加。在整个研究中，HS181 hESC系在非活性HFF上以未分化状态生长。

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