• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Flow cytometric sorting and analysis of human epidermal stem cell candidates.
【24h】

Flow cytometric sorting and analysis of human epidermal stem cell candidates.

机译：人表皮干细胞候选者的流式细胞仪分选和分析。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

ESC (epidermal stem cells) play a central role in the regeneration of human epidermis. These cells are also responsible for wound healing and neoplasm formation. Efficient isolation of ESC allows their use in medicine and pharmacy as well as in basic science. Cultured keratinocytes and ESC may be used as biological dressing in burn injuries, chronic wounds and hereditary disorders. Therefore, the isolation and characterization of ESC have been goals in biomedical science. Here, we present a flow cytometric method for the isolation and analysis of human ESC candidates. The strategy presented for the isolation of ESC combines previously proposed enzymatic digestion and FACS-sorting of the obtained cell suspension that utilizes morphological features, integrin-beta1 expression and Rh123 (Rhodamine 123) accumulation of the cells. We also performed a flow cytometric analysis of sorted cells using a cell tracer.
机译：ESC（表皮干细胞）在人类表皮的再生中起着核心作用。这些细胞还负责伤口愈合和肿瘤形成。 ESC的有效隔离使其可以在医学和药学以及基础科学中使用。培养的角质形成细胞和ESC可用作烧伤，慢性伤口和遗传性疾病的生物敷料。因此，ESC的分离和表征一直是生物医学领域的目标。在这里，我们提出了一种用于人类ESC候选者的分离和分析的流式细胞仪方法。提出的分离ESC的策略结合了先前提出的酶消化和获得的细胞悬浮液的FACS分选，利用了细胞的形态特征，整联蛋白β1表达和Rh123（Rhodamine 123）积累。我们还使用细胞示踪剂对分选的细胞进行了流式细胞仪分析。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号