• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Ginsenosides promote proliferation of granulosa cells from chicken prehierarchical follicles through PKC activation and up-regulated cyclin gene expression.
【24h】

Ginsenosides promote proliferation of granulosa cells from chicken prehierarchical follicles through PKC activation and up-regulated cyclin gene expression.

机译：人参皂甙可通过PKC激活和上调细胞周期蛋白基因表达来促进鸡前卵泡中颗粒细胞的增殖。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effect of GS (ginsenosides) on proliferation of chicken GCs (granulosa cells) from prehierarchical SYF (small yellow follicles) was evaluated, and involvement of the PKC (protein kinase C) signalling pathway as well as mRNA expression of cyclins and CDK (cyclin-dependent kinase) were investigated. Whole SYF or GCs isolated from SYF were cultured in Medium 199 supplemented with 0.5% FCS (fetal calf serum). After 16 h, the cells were challenged with GS alone or in combination with PKC inhibitor H7 or activator PMA (phorbol 12-myristate 13-acetate) for 24 h in serum-free medium. Results showed that in both whole follicles and pure GCs monolayer culture system, GS (0.1-10 microg/ml) significantly increased the number of GCs in SYF in a dose-dependent manner, and this stimulatory effect was inhibited by H7, but enhanced by PMA. Meanwhile, the PCNA-LI (proliferating cell nuclear antigen labelling index) of GCs displayed similar changes with the cell number. Mechanism of GS action was further evaluated in cultured GCs separated from SYF. Western blot analysis showed that 10 microg/ml GS increased PKC translocation from cytoplasm to the plasma membrane of the GCs to become the active state. This effect was blocked by H7. Furthermore, GS up-regulated the expression of cyclin D1/CDK6 and cyclin E/CDK2 mRNAs in GCs; however, inhibition of PKC with H7 attenuated this stimulatory effect. These results indicated that GS could stimulate proliferation of chicken GCs through activated PKC-involved up-regulation of cyclin D1/CDK6 and cyclin E/CDK2 genes, subsequently promoting development of the chicken prehierarchical follicles.
机译：评估了GS（人参皂甙）对鸡GC（颗粒状小卵泡）中GC（颗粒细胞）增殖的影响，并参与了PKC（蛋白激酶C）信号通路的参与以及cyclins和CDK（cyclin的mRNA）的表达-依赖性激酶）进行了研究。从SYF中分离出的整个SYF或GC在添加有0.5％FCS（胎牛血清）的199培养基中培养。 16小时后，在无血清培养基中将细胞单独或与PKC抑制剂H7或激活剂PMA（佛波12-肉豆蔻酸酯13-乙酸酯）联合用GS攻击24小时。结果显示，在整个卵泡和纯GC单层培养系统中，GS（0.1-10 microg / ml）均以剂量依赖性方式显着增加SYF中GC的数量，这种刺激作用被H7抑制，但被H7增强。 PMA。同时，GC的PCNA-LI（增殖细胞核抗原标记指数）显示出与细胞数量相似的变化。在从SYF分离的培养的GC中进一步评估了GS作用的机制。蛋白质印迹分析表明，10 µg / ml GS增加了PKC从细胞质到GC质膜的转运，成为活性状态。 H7阻止了此效果。此外，GS上调了GC中cyclin D1 / CDK6和cyclin E / CDK2 mRNA的表达。然而，用H7抑制PKC减弱了这种刺激作用。这些结果表明，GS可以通过激活PKC参与的细胞周期蛋白D1 / CDK6和细胞周期蛋白E / CDK2基因的上调刺激刺激鸡GC的增殖，从而促进鸡前毛囊的发育。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号