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首页> 外文期刊>Cell biology international. >Influence of protein histidine phosphatase overexpression and down-regulation on human umbilical-vein endothelial cell viability
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Influence of protein histidine phosphatase overexpression and down-regulation on human umbilical-vein endothelial cell viability

机译：蛋白组氨酸磷酸酶的过表达和下调对人脐静脉内皮细胞活力的影响

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摘要

PHP (protein histidine phosphatase) is expressed by mammalian tissues, particularly in blood vessel walls. We investigated whether PHP plays a significant role in endothelial cells. By Western blot and immunofluorescence analysis PHP was found in HUVEC (human umbilical-vein endothelial cells). Overexpression of PHP by the use of a plasmid vector, pIRES2-AcGFP1-PHP, induced apoptosis in HUVEC. To exclude the possibility that increased cellular protein alone unspecifically caused cell damage, the inactive H53A mutant of PHP was also overexpressed as a control; it did not lead to apoptosis. Down-regulation of PHP by the RNAi (RNA interference) technique did not affect cell viability. In conclusion, HUVEC are damaged by overexpression, but not down-regulation, of PHP, suggesting a pronounced impact of the enzyme on the cells when its activity is increased.
机译：PHP（蛋白质组氨酸磷酸酶）由哺乳动物组织表达，尤其是在血管壁中。我们调查了PHP是否在内皮细胞中起重要作用。通过Western印迹和免疫荧光分析，在HUVEC（人脐静脉内皮细胞）中发现了PHP。通过使用质粒载体pIRES2-AcGFP1-PHP过度表达PHP，可诱导HUVEC凋亡。为了排除单独增加的细胞蛋白非特异性引起细胞损伤的可能性，PHP的无活性H53A突变体也被过表达作为对照。它没有导致细胞凋亡。通过RNAi（RNA干扰）技术对PHP的下调不会影响细胞活力。总之，HUVEC被PHP的过表达所破坏，而不受PHP下调的损害，这表明当酶的活性增加时，酶对细胞的影响显着。

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