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首页> 外文期刊>Cell biology international. >Functional examination of microencapsulated bioengineered insulin-secreting beta-cells.
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Functional examination of microencapsulated bioengineered insulin-secreting beta-cells.

机译：微囊化的生物工程胰岛素分泌β细胞的功能检查。

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摘要

Clonal insulin-secreting BRIN-BD11 cells engineered by electrofusion were encapsulated inside natrium alginate beads and cultured in RPMI 1640 culture media. Acute insulin secretory responses to glucose and amino acids were compared between microencapsulated cells and non-encapsulated cells maintained in monolayer culture. Encapsulated cells exhibited a 1.5-fold, 2.9-fold and 4.2-fold increase (P< 0.001) in insulin release in response to 16.7 mmol/l glucose, 10 mmol/l L-arginine and 10 mmol/l L-alanine respectively. Insulin output by non-encapsulated cells was approximately 30% greater but the relative magnitudes of responses were similar. This is the first study to demonstrate the stability of cellular engineered insulin-secreting cells encapsulated in alginate beads, illustrating the utility of this approach for cellular engineering and potential transplantation in diabetes. Copyright 2001 Academic Press.
机译：通过电融合工程改造的克隆分泌胰岛素的BRIN-BD11细胞被封装在海藻酸钠微珠内，并在RPMI 1640培养基中培养。比较了微囊化细胞和单层培养中维持的非囊化细胞之间对葡萄糖和氨基酸的急性胰岛素分泌反应。封装的细胞分别响应于16.7 mmol / l葡萄糖，10 mmol / l L-精氨酸和10 mmol / l L-丙氨酸，胰岛素释放表现出1.5倍，2.9倍和4.2倍的增加（P <0.001）。非封装细胞的胰岛素输出量大约高出30％，但响应的相对幅度相似。这是第一项证明藻酸盐微珠中包裹的细胞工程胰岛素分泌细胞的稳定性的研究，说明了该方法在糖尿病中进行细胞工程和潜在移植的实用性。版权所有2001，学术出版社。

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