Carcinogenesis or tumourigenicity testing of animal cell lines for vaccine preparation by colony formation on soft agar and by agglutination under plant lectins.

Zhang DL; Liu SG; Yan LF; Li LJ; Huang GS; Fang FD; Xia GT; He XY; Gao BX; Bai XH; Wang W; Ding PG

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Carcinogenesis or tumourigenicity testing of animal cell lines for vaccine preparation by colony formation on soft agar and by agglutination under plant lectins.

机译：通过在软琼脂上形成菌落并在植物凝集素下凝集来测试动物细胞系的致癌性或致瘤性，以制备疫苗。

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The carcinogenic or tumourigenic testing of seven animal kidney cell lines (F-81, CRFK, MDCK, Vero, Vero-2 cell line, MA-104 and BHK-21) established in China, were carried out in more than 700 nude mice for colony formation in soft agar and for agglutination under different density of plant lectins. Tests showed that there were correlation between cell line chromosome number variations and anchorage independence in soft agar, agglutinability under lectins and tumour-forming ability in nude mice. Since testing in vitro was more economical, simpler and faster and thus thought to be more reliable, we recommend measuring agglutinability, followed by anchorage independence or analysis of karyotype as the initial means for monitoring tumourigenicity of animal cell lines in nude mice.

机译：在中国建立的7种动物肾细胞系（F-81，CRFK，MDCK，Vero，Vero-2细胞系，MA-104和BHK-21）的致癌或致瘤性试验在700多只裸鼠中进行，在软琼脂上形成菌落，并在不同密度的植物凝集素下凝集。实验表明，细胞株染色体数目变异与软琼脂的锚定独立性，凝集素下的凝集性和裸鼠的肿瘤形成能力之间存在相关性。由于体外测试更经济，更简单，更快，因此被认为更可靠，因此我们建议先测量凝集性，然后进行锚定独立性或核型分析，作为监测裸鼠动物细胞系致瘤性的初始方法。

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《Cell biology international.》 |2001年第10期|共6页
作者
Zhang DL; Liu SG; Yan LF; Li LJ; Huang GS; Fang FD; Xia GT; He XY; Gao BX; Bai XH; Wang W; Ding PG;
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正文语种 eng
中图分类细胞生物学;
关键词
Agglutination; Carcinogenicity Tests; Lectins; Tumor Stem Cell Assay; 凝集; 致癌性试验; 外源凝集素类; 肿瘤干细胞测定;

机译：Agglutination;Carcinogenicity Tests;Lectins;Tumor Stem Cell Assay;凝集;致癌性试验;外源凝集素类;肿瘤干细胞测定;

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专利

1. Carcinogenesis or tumourigenicity testing of animal cell lines for vaccine preparation by colony formation on soft agar and by agglutination under plant lectins. [J] . Zhang DL, Liu SG, Yan LF, Cell biology international. . 2001,第10期

机译：通过在软琼脂上形成菌落并在植物凝集素下凝集来测试动物细胞系的致癌性或致瘤性，以制备疫苗。
2. The effect of overexpression of the protein tyrosine phosphatase PTPMEG on cell growth and on colony formation in soft agar in COS-7 cells [J] . Minxiang Gu, Kun Meng, Philip W. Majerus Proceedings of the National Academy of Sciences of the United States of America . 1996,第23期

机译：酪氨酸磷酸酶PTPMEG蛋白过表达对COS-7细胞中琼脂细胞生长和集落形成的影响
3. Ultra-sensitive detection of tumorigenic cellular impurities in human cell-processed therapeutic products by digital analysis of soft agar colony formation [J] . Shinji Kusakawa, Satoshi Yasuda, Takuya Kuroda, Scientific reports. . 2015,第1期

机译：通过软琼脂菌落形成的数字分析超灵敏地检测人细胞加工的治疗产品中的致瘤细胞杂质
4. Application of animal-free recombinant bioactive proteins to improve the growth performance of cell lines for viral vaccines [C] . Marina Ross, Domenica Cavallaro, Larissa Chirkova, Vaccine Technology Symposium . 2010

机译：无动物重组生物活性蛋白的应用改善病毒疫苗细胞系生长性能
5. Chimeric orthohepadnavirus core particles for oral delivery of vaccines: Part I. Transformation of tobacco plants with a gene encoding a c-terminus truncated hepatitis B virus core protein. Part II. Construction of a woodchuck hepatitis virus core protein-based universal epitope carrier and test expression in Escherichia coli. [D] . Callaghan, Maximilian W. 2001

机译：用于口服递送疫苗的嵌合正丙肝病毒核心颗粒：第一部分。用编码c末端截短的乙型肝炎病毒核心蛋白的基因转化烟草植物。第二部分基于土拨鼠肝炎病毒核心蛋白的通用表位载体的构建和大肠杆菌中的表达测试。
6. Expression of the E4 gene is required for establishment of soft-agar colony-forming rat cell lines transformed by the adenovirus 12 E1 gene. [O] . K Shiroki, S Hashimoto, I Saito, 1984

机译：E4基因的表达是建立由腺病毒12 E1基因转化的软琼脂集落形成大鼠细胞系所必需的。
7. EP18.12: To compare the use of single planes versus z-stack volumes in the assessment of tumourigenicity of HeLa cells with soft agar assay [O] . H.S. Wong, E.T.K. Lau, H.Y.M. Tang, 2018

机译：EP18.12：将单个平面与Z-Stack卷的使用进行比较评估Hela细胞与软琼脂测定的肿瘤

Carcinogenesis or tumourigenicity testing of animal cell lines for vaccine preparation by colony formation on soft agar and by agglutination under plant lectins.

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