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首页> 外文期刊>Cell and Tissue Research >Transmigration of macrophages across the choroid plexus epithelium in response to the feline immunodeficiency virus
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Transmigration of macrophages across the choroid plexus epithelium in response to the feline immunodeficiency virus

机译：响应猫免疫缺陷病毒，巨噬细胞跨脉络丛上皮迁移。

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Although lentiviruses such as human, feline and simian immunodeficiency viruses (HIV, FIV, SIV) rapidly gain access to cerebrospinal fluid (CSF), the mechanisms that control this entry are not well understood. One possibility is that the virus may be carried into the brain by immune cells that traffic across the blood-CSF barrier in the choroid plexus. Since few studies have directly examined macrophage trafficking across the blood-CSF barrier, we established transwell and explant cultures of feline choroid plexus epithelium and measured trafficking in the presence or absence of FIV. Macrophages in co-culture with the epithelium showed significant proliferation and robust trafficking that was dependent on the presence of epithelium. Macrophage migration to the apical surface of the epithelium was particularly robust in the choroid plexus explants where 3-fold increases were seen over the first 24 h. Addition of FIV to the cultures greatly increased the number of surface macrophages without influencing replication. The epithelium in the transwell cultures was also permissive to PBMC trafficking, which increased from 17 to 26%of total cells after exposure to FIV. Thus, the choroid plexus epithelium supports trafficking of both macrophages and PBMCs. FIV significantly enhanced translocation of macrophages and T cells indicating that the choroid plexus epithelium is likely to be an active site of immune cell trafficking in response to infection.
机译：尽管诸如人，猫和猿猴免疫缺陷病毒（HIV，FIV，SIV）之类的慢病毒迅速获得了脑脊液（CSF）的访问权限，但控制这种进入的机制仍未得到很好的理解。一种可能性是病毒可以通过跨脉络丛中血液-CSF屏障的免疫细胞带入大脑。由于很少有研究直接检查跨血脑脊液屏障的巨噬细胞运输，因此我们建立了猫脉络丛上皮的transwell和外植体培养，并在有或没有FIV的情况下测量了运输。与上皮细胞共培养的巨噬细胞显示出明显的增殖和强大的运输，这取决于上皮细胞的存在。在脉络丛外植体中，巨噬细胞向上皮顶表面的迁移特别牢固，在最初的24小时内观察到其增加了3倍。在培养物中加入FIV大大增加了表面巨噬细胞的数量，而不会影响复制。跨孔培养中的上皮细胞也允许PBMC转运，暴露于FIV后，PBMC转运从占总细胞的17％增加到26％。因此，脉络丛上皮支持巨噬细胞和PBMC的运输。 FIV显着增强了巨噬细胞和T细胞的转运，表明脉络丛上皮很可能是免疫细胞运输对感染作出反应的活性部位。

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