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首页> 外文期刊>Cell and Tissue Research >Leptin-sensitive OBP-expressing mucous cells in rat olfactory epithelium: a novel target for olfaction-nutrition crosstalk?
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Leptin-sensitive OBP-expressing mucous cells in rat olfactory epithelium: a novel target for olfaction-nutrition crosstalk?

机译：大鼠嗅觉上皮中表达瘦素的OBP表达黏膜细胞：嗅觉营养串扰的新靶点吗？

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Although odorant-binding proteins (OBP) are one of the most abundant classes of proteins in the mammalian olfactory mucus, they have only recently been ascribed a functional role in the detection of odorants by olfactory neurons. Among the three OBPs described in the rat, OBP-1f is mainly secreted by the lateral nasal glands (LNG) and Bowman's glands, and its expression is transcriptionally regulated by food deprivation in the olfactory mucosa, but not in LNG. Therefore, mucus composition might be locally regulated by hormones or molecules relevant to nutritional status. Our aim has been to investigate the mechanisms of such physiological regulation at the cellular level, through both the examination of OBP-1f synthesis sites in the olfactory mucosa and their putative regulation by leptin, a locally acting satiety hormone. Immunohistochemical observations have allowed the identification of a novel population of OBP-1f-secreting cells displaying morphological and functional characteristics similar to those of epithelial mucous cells. Ultrastructural analyses by both transmission and scanning electron microscopy has enabled a more complete cytoarchitectural characterization of these specialized olfactory mucous cells in their tissue environment. These globular cells are localized in discrete zones of the olfactory epithelium, mainly in the fourth turbinate, and are often scattered from the basal to the apical surface of the epithelium. They contain numerous small droplets of mucosubstances. Using an in-vitro-derived model of olfactory mucosa primary culture, we have been able to demonstrate that leptin increases the production of mucus by these cells, so that they constitute potential targets for the physiological modulation of mucus composition by nutritional cues.
机译：尽管加味剂结合蛋白（OBP）是哺乳动物嗅觉粘液中最丰富的蛋白之一，但直到最近才将它们归因于嗅觉神经元在检测味剂中的功能性作用。在大鼠中描述的三种OBP中，OBP-1f主要由鼻外侧腺（LNG）和Bowman腺分泌，其表达受嗅觉粘膜食物剥夺而转录调控，而在LNG中不受转录控制。因此，粘液成分可能受与营养状况有关的激素或分子的局部调节。我们的目标是通过检查嗅觉粘膜中的OBP-1f合成位点以及通过局部作用的饱腹激素leptin进行的假定调节，来研究在细胞水平上这种生理调节的机制。免疫组织化学观察已允许鉴定新的OBP-1f分泌细胞群，这些细胞表现出与上皮黏液细胞相似的形态和功能特征。通过透射电子显微镜和扫描电子显微镜进行的超微结构分析已经使这些特殊的嗅觉粘液细胞在其组织环境中得到了更完整的细胞结构表征。这些球状细胞位于嗅觉上皮的离散区域，主要在第四鼻甲中，并且通常从上皮的基底表面扩散到顶表面。它们包含许多粘液物质小滴。使用嗅觉粘膜原代培养的体外模型，我们已经能够证明瘦素增加了这些细胞产生的粘液，因此它们构成了通过营养线索对粘液成分进行生理调节的潜在靶标。

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