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首页> 外文期刊>Cell and Tissue Research >Localization of hematopoietic cells in the bullfrog (Lithobates catesbeianus).
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Localization of hematopoietic cells in the bullfrog (Lithobates catesbeianus).

机译：牛蛙（Lithobates catesbeianus）中的造血细胞定位。

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Amphibians represent the first phylogenetic group to possess hematopoietic bone marrow. However, adult amphibian hematopoiesis has only been described in a few species and with conflicting data. Bone marrow, kidney, spleen, liver, gut, stomach, lung, tegument, and heart were therefore collected from adult Lithobates catesbeianus and investigated by light microscopy and immunohistochemical methods under confocal laser microscopy. Our study demonstrated active hematopoiesis in the bone marrow of vertebrae, femur, and fingers and in the kidney, but no hematopoietic activity inside other organs including the spleen and liver. Blood cells were identified as a heterogeneous cell population constituted by heterophils, basophils, eosinophils, monocytes, erythrocytic cells, lymphocytes, and their precursors. Cellular islets of the thrombocytic lineage occurred near sinusoids of the bone marrow. Antibodies against CD34, CD117, stem cell antigen, erythropoietin receptor, and the receptor for granulocyte colony-stimulating factor identified some cell populations, and some circulating immature cells were seen in the bloodstream. Thus, on the basis of these phylogenetic features, we propose that L. catesbeianus can be used as an important model for hematopoietic studies, since this anuran exhibits hematopoiesis characteristics both of lower vertebrates (renal hematopoiesis) and of higher vertebrates (bone marrow hematopoiesis).
机译：两栖动物是拥有造血骨髓的第一个系统发育群体。但是，成年两栖造血功能仅在少数物种中有描述，并且数据相互矛盾。因此，从成年的Litesbates catesbeianus收集了骨髓，肾脏，脾脏，肝脏，肠胃，胃，肺，皮被和心脏，并在共聚焦激光显微镜下通过光学显微镜和免疫组化方法进行了研究。我们的研究表明，在椎骨，股骨和手指的骨髓中以及在肾脏中有活跃的造血功能，但在包括脾脏和肝脏在内的其他器官内部却没有造血活动。血细胞被鉴定为异源性细胞群，由异嗜性，嗜碱性粒细胞，嗜酸性粒细胞，单核细胞，红细胞，淋巴细胞及其前体组成。血小板谱系的细胞胰岛发生在骨髓的正弦曲线附近。针对CD34，CD117，干细胞抗原，促红细胞生成素受体和粒细胞集落刺激因子受体的抗体鉴定了一些细胞群，并且在血流中发现了一些循环中的未成熟细胞。因此，基于这些系统发育特征，我们建议将L. catesbeianus用作造血研究的重要模型，因为该无核小动物既表现出较低脊椎动物（肾脏造血）又表现出较高脊椎动物（骨髓造血）的造血特征。 。

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